生地黄不同炮制阶段寡糖和梓醇的变化研究-天津中草药杂志社.pdf

·2434 · 中草药 Chinese Traditional and Herbal Drugs 第 42 卷 第 12 期 2011 年 12 月 生地黄不同炮制阶段寡糖和梓醇的变化研究 * 邱建国，张汝学，贾正平 ，李茂星，张泉龙，周 珺，尉丽力 兰州军区兰州总医院 药材科，全军高原环境损伤防治重点实验室，甘肃 兰州 730050 摘 要：目的 考察生地黄不同炮制阶段所含地黄寡糖和梓醇量的变化。方法 采用水为溶媒，超声提取不同炮制阶段生地 黄中寡糖类成分，流动相用乙腈-水（72 ∶28 ），体积流量 1.0 mL/min，NH2 色谱柱分离，示差折光检测器检测，HPLC 法测 定；采用甲醇为溶媒，超声提取梓醇，流动相用甲醇-0.1%磷酸溶液（1 ∶99 ），体积流量 1.0 mL/min，C18 色谱柱分离，检测 波长 210 nm，HPLC 法测定。结果 不同炮制阶段生地黄中的寡糖成分及其量不同，梓醇的量不同。结论 炮制改变了生 地黄中寡糖成分类型及其量，同时也影响梓醇的量。 关键词：炮制；生地黄；寡糖；梓醇；HPLC 中图分类号：R286.1 文献标志码：A 文章编号：0253 - 2670(2011)12 - 2434 - 04 Change of oligosaccharide and catalpol contents in Rehmanniae Radix at various processing phases QIU Jian-guo, ZHANG Ru-xue, JIA Zheng-ping, LI Mao-xing, ZHANG Quan-long, ZHOU Jun, WEI Li-li Department of Pharmacy, Lanzhou General Hospital of PLA, Key Laboratory of the Prevention and Cure for the Plateau Environment Damage, PLA, Lanzhou 730050, China Abstract: Objective To investigate the contents of oligosaccharide and catalpol in Rehmanniae Radix at various processing phases. Methods Saccharide ingredient was extracted by ultrosonic extraction when water was used as solvent, separated by NH2 chromatographic column while acetonitrile-water (72 ∶28) as mobile phase at flow rate of 1.0 mL/min, detected by differential refractive detector, and assayed by HPLC. Catalpinol was extracted by ultrosonic extraction when methanol was used as solvent, separated by C （ ） 18 chromatographic column while methanol-0.1% phosphoric acid 1 ∶99 as mobile phase at flow rate of 1.0 mL/min, detected at wavelength of 210 nm, and assayed by HPLC. Results There are significantdifferences at various processing phases of oligosaccharide and catalpol contents in