生鲜肉中牛源性和羊源性成分定量检测方法的建立-食品安全质量检测.PDF

7 3 Vol. 7 No. 3 2016 3 Journal of Food Safety and Quality Mar. , 2016 冯 震, 杨美成* ( , 201203) 摘 要: 目的 PCR 方法 b(Cytb) , , SYBR PCR , 结果 60 ℃, PCR , Ct 20, Ct 35, 0.016~10 ng/μL ; Ct30 , PCR 1%结论 关键词: ; ; PCR; b Establishment of quantitative methods for detecting bovine-derived and ovine-derived materials in fresh meat * FENG Zhen, YANG Mei-Cheng (Shanghai Institute for Food and Drug Control, Shanghai 201203, China) ABSTRACT: Objective To establish a method for detection of bovine-derived and ovine-derived materials in fresh meat by fluorescence quantitative PCR. Methods Fluorescence quantitative PCR method based on SYBR was established with cytochrome b nucleotide sequence from mammal’s plastochondria genome as target. Fluorescence quantitative PCR primers were designed and synthesized and the specificity and sensitivity of this method was respectively performed for validation. Results Primers for fluorescence quantitative PCR reaction had good specificity under the condition of annealing temperature 60 ℃, Ct values of target gene signals were less than 20, and Ct values of nonspecific signals were more than 35. This method had a good linearity in the range of 0.016~10 ng/μL.