细胞内蛋白质的分选和运输.ppt

Arthrogryposis(关节弯曲 ) Renal dysfunction and Cholestasis(胆汁郁积) (ARC) syndrome most platelets seen in ARC syndrome virtually lack recognizable a granules. Only empty vacuoles (v) as the remnants of a granules that have lost their granular contents are observed (C, D). There are also hypogranular platelets in patients with ARC syndrome (E, F). No notable abnormalities are found in other organelles such as mitochondria and d granules. actin merge beta3 Pitstop2 5uM DMSO 90min * * Each of them contain a unique set of proteins . They carries out a specialized function * * Synaptic vesicle recycling pathways. Overview of vesicular transport Transition vesicles Anterograde Transport Constituitive Regulated Exocytosis secretion Early Endosome Lysosome Late Endosome Endocytosis Retrograde Transport Figure 15-18 Essential Cell Biology (? Garland Science 2010) How vesicles shuttle proteins and membranes between intracellular compartments? How does a vesicle find its way to its correct destination to deliver its contents? Figure 15-19a Essential Cell Biology (? Garland Science 2010) Vesicle budding is driven by the assembly of a protein coat Coat proteins: curve membrane concentrate proteins rough ER cis trans medial golgi COPII COPI clathrin plasma membrane Assembly of a protein coat drives vesicle formation and selection of cargo molecules Targeting sequences on cargo protein make specific molecular contacts with coat proteins. clathrin coated vesicles Model of Clatrin-coated vesicle budding Lodish 17-20 （coated vesicle） Figure 15-18 Essential Cell Biology (? Garland Science 2010) How does a vesicle find its way to its correct destination to deliver its contents? In most case,the vesicle is actively transported by motor proteins that move along cytoskeletal fibers. Figure 15-21 Essential Cell Biology (? Garland Science 2010) Rab roteins and SNAREs helpdirect transport vesicles to their target membranes Paired sets of SNARE proteins mediate fusion of ves