环磷酰胺致大鼠卵巢功能不全建立卵巢移植供体模型的研究.doc

环磷酰胺致大鼠卵巢功能不全建立卵巢移植供体模型的研究 丁岩1，邵佳亮2， 李珺玮1，张英1，华克勤1,3，王翔2,3 复旦大学附属妇产科医院妇科，上海 200090 上海市第一人民医院泌尿外科，上海 200080 通讯作者：华克勤：huakeqinjiaoshou@163.com; 王翔：drseanwang@163.com 基金项目：国家重点基础研究发展计划（973计划，2011CB944503） 【摘要】目的 探索建立化疗所致卵巢功能损伤大鼠模型的适宜方法和最佳时间，为后续卵巢移植治疗提供实验基础。方法 60只成熟雌性Lewis大鼠随机分为对照组（生理盐水组）、低剂量组（环磷酰胺6mg/kg）、高剂量组（环磷酰胺8mg/kg）， 分别于化疗前、化疗结束、化疗结束后1周、化疗结束后2周观察大鼠一般情况、动情周期、血清性激素水平及卵巢组织形态学改变，评估大鼠状态及卵巢功能，通过检测血氨基转移酶水平评估肝功能。 结果 化疗后大鼠一般情况差，氨基转移酶升高，动情周期紊乱，血激素水平显著下降（P0.01）。化疗后2周大鼠体重仍显著低于对照组（P0.05），但进食、活动好，氨基转移酶正常；动情周期未恢复正常（P0.05）；卵巢各级卵泡明显减少（P0.05）；与对照组相比，高剂量组血激素水平仍显著降低（P0.05），而低剂量组无明显差异（P0.05）。 结论 以环磷酰胺高剂量组腹腔化疗可建立理想的卵巢功能损伤大鼠模型，并持续至化疗后2周，适于做卵巢移植供体。 【关键词】 环磷酰胺，卵巢功能损伤，化疗，动物模型，大鼠 【中图分类号】R711.75 【文献标识码】A 【文章编号】 Establishment of ovarian transplantation rat model of premature ovarian insufficiency induced by cyclophosphamide Yan Ding 1, Jia-liang Shao2, Jun-wei Li1,Ying Zhang1, Ke-qin Hua1,3, Xiang Wang2,3 1 Department of Gynecology, Obstetrics and Gynecology Hospital, Fudan University, Shanghai, 200090, China 2 Department of Urology, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200080, China [Abstract] Objective To investigate an applicable method and optimal time in establishment of rat model of cyclophosphamide(CTX)-induced premature ovarian insufficiency (POI), which is opt to treatment by whole ovarian transplantation. Methods 60 female Lewis rats were divided into three groups: control group (with saline), low-dose group (CTX 6mg/kg) and high-dose group (CTX 8mg/kg). The changes of general state, estrous cyclicity, gonadal hormones, aminotranferases levels and pathological changes were observed. Results CTX induced decreased body, abnormal estrous cyclicity, decreased hormone levels and lower aminotranferases levels after treatment（P0.05）. After two weeks of CTX treatment, the rats recovered physically, but the ovarian function in high-dose group were still significantly lower than that in control group (P0.05), while no significant difference