自制人血清中期角膜保存液对兔角膜保存的实验研究.doc

自制人血清中期角膜保存液对兔角膜的保存效果的实验研究 杨 扬,周善璧,陈小雄,刘珍珍(400016 重庆，重庆医科大学附属第一医院眼科，；重庆市眼科学重点实验室) [摘要] 目的 研制一种适合我国国情的人血清角膜中期保存液。方法 配制人血清浓度分别为10%(A组)、20%(B组)、30%(C组)、0%(D组)的角膜保存液保存兔角膜，用Optisol-GS角膜保存液作对照组，分别在保存第3、7、10、14天进行角膜内皮细胞活性率检测、HE染色、扫描电镜超微结构观察，并检测角膜保存液的理化性质及生物安全性。结果 兔角膜保存至第14天时，C组角膜内皮细胞活性率与对照组比较差异无明显统计学意义（P＞0.05），被保存的角膜组织病理学和超微结构C组与对照组Optisol-GS无显著差异。且血清角膜保存液理化性质稳定，无生物危险性。A、B、D组与对照组比较，角膜内皮细胞活性率分别在保存第7、10、3应是从小到大排列？天时差异具有明显的统计学意义（P＜0.05）；角膜组织病理学比较，在保存第7天，各实验组与对照组无明显差异（P0.05）只是病理学观察，做了统计学分析吗？没做统计学分析就不要写P0.05，随着时间延长，D、A、B组先后依次出现组织病理学改变；超微结构比较，在保存第7天时，D组与C组及对照组具有明显差异。结论 30%人血清角膜保存液具有良好的兔角膜保存效果，其理化性质稳定且安全, 可保存兔角膜至7~10 d。 应是从小到大排列？ 只是病理学观察，做了统计学分析吗？没做统计学分析就不要写P0.05 [关键词]角膜保存液；人血清；兔角膜，角膜内皮细胞 [中图法分类号]R77; R779.6; R779.65 [文献标识码] A A report on investigation of corneal storage medium containing human autologous serum for rabbits’ cornea. Yang Yang, Zhou Shanbi, Chen Xiaoxiong, Liu Zhenzhen (Department of Ophthalmology, First Affiliated Hospital of Chongqing Medical University; Chongqing Key Laboratory of Ophthalmology，Chongqing, 400016, China) [Abstract ] Objective To develop a mid-term corneal storage medium containing autologous serum for Chinese conditions. Methods Cornea of rabbits were stored in experimental groups A,B,C,D corneal storage medium containing human autologous serum and control group Optisol-GS. Autologous serum(10%, 20%, 30%, 0%) were added into experimental groups A,B,C,D respectively. At the 3rd,7th ,10th,14th day after preservation, the viability of corneal endothelial cells was detected by trypan blue—alizarin red staining，tissue structure was detected by pathological section of cornea, the ultramicroscopic structure of corneal endothelial cells was observed by scanning electron microscope. The physicochemical property and biological safety of corneal storage medium was detected by microbiological evaluation. Results At the 14th day after preservation, there was no significant difference between group C and Optisol-GS on the viability of corneal endoth