平肺口服液抑制肺成纤维细胞生长和诱导凋亡课件.pptVIP

* 平肺口服液抑制肺成纤维细胞生长和诱导凋亡 研究背景 放射性肺炎及肺纤维化是胸部放疗中部分正常肺组织因受到一定剂量射线照射而发生的放射损伤 肺成纤维细胞是射线作用的效应细胞之一。它在射线作用下发生活化和增殖，持续合成并分泌细胞因子，从而导致胶原代谢调控失衡和肺组织纤维化 Imbalanced wound-healing response* *Wilson MS, Wynn TA. Pulmonary fibrosis: pathogenesis, etiology and regulation. Mucosal Immunol. 2009 Mar;2(2):103-21. 2 平肺口服液是中日友好医院药学部中药复方制剂，该方源自中西医结合肿瘤专家李佩文教授经验方，能用于放射性肺炎的辩证论治1。治疗原则：养阴清肺，解毒散结 实验研究表明，平肺口服液能有效预防大鼠放射性肺损伤2 1 程志强，张嘉，刘轩等. 平肺口服液在非小细胞肺癌放疗中的应用. 中日友好医院学报,2009,23(6):334-336 2 刘轩，程志强，徐波等. 平肺口服液对放射性肺炎大鼠肺组织细胞因子的调节作用. 中国免疫学杂志,2009,25(9):808-812 3 A B C D E HE staining in lung tissue of rats after a single whole thoracic irradiation with a dose of 20Gy A: Normal control; B: Model group, 4 weeks after irradiation; C: Model group, 8 weeks after irradiation; D: Pingfei group, 4 weeks after irradiation; E: Pingfei group, 8 weeks after irradiation. 4 ** # ** ## ** ## mRNA of IL-6,IL-10 and IFN-γ expression in the rat lung tissue 4 weeks after a single whole thoracic irradiation. Note: Total RNA from the lung tissue was harvested at the 4th week after a dose of 20Gy irradiation in rat whole thoraces. mRNA of IL-6,IL-10 and IFN-γ expression was assayed by RT-PCR. Products of RT-PCR were electrophoresed in 2% agarose gel in the presence of ethidium bromide（80V,20min）,with the same sample’s beta-actin as internal control. The ratios of target gene vs internal control were calculated, and the results were expressed as mean±standard deviation（mean±sd）.The intensities of the specific bands were analysed using the software Quantity One 4.4.0(Bio-Rad) to determine whether the differences were statistically significant. Compared with Control group, **. P0.01; Compared with Model group, ##. P0.01; Compared with Model group, #. P0.05. 5 研究目的 采用血清药理学方法，研究平肺口服液对人胚肺成纤维细胞增殖及诱导细胞凋亡的作用。 6 材料与方法 平肺口服液10 g/kg或20 g/kg灌服SD大鼠 每日一次，连续7 d 第8－10d，每日两次，两次灌服间隔12 h 第10d晚上灌服后禁食不禁水 第11d上午末次灌服后2 h 无菌腹主动脉取血 4oC静置6h，离心，取上清，分装，-20oC冻存 平肺口服液药物组成：百合15 g，麦冬15 g，五味子10 g，白及10 g，瓜蒌10 g，鱼腥草30 g，白花蛇舌草30 g，桑白皮30 g，浙贝母10