不同纳米碳浓度对细胞生长的影响-第三军医大学学报.doc

石墨碳纳米颗粒对体外培养细胞数量及超微结构的影响 刘东京 刘美洲 张阳德 刘东非 潘一峰 陈 伟 刘 辉 曾庆仁 张红* （中南大学湘雅医院卫生部肝胆肠外科研究中心 长沙 410008） 摘　要: 目的 了解石墨碳纳米颗粒(carbon nanoparticles，CNP)的形态特征，观察CNP其对体外培养细胞增殖与超微结构的影响。方法 用原子粒显微镜和电镜观察分析CNP 的大小、形状。选择人肝细胞株（L02）、人肝细胞株（Hl7702）、小鼠细胞株（3T3）和人肝癌细胞株（HepG2）作为观察对象，在体外培养中，将每种细胞株按设计添加不同浓度CNP，每个浓度设3孔。用血细胞计数板计数法测定细胞数量，透射电镜观察细胞超微结构，通过方差分析和Dunnett检验作统计学处理。结果 CNP 约为20nm的球形微粒。添加有不同浓度CNP 的4株细胞各实验组，除HepG2细胞株外，其他三组细胞数量水平均显著多于未添加CNP 的细胞对照组，其中以7.5μg/ml浓度组最为突出。透射电镜观察添加浓度为7.5μg/ml CNP 的细胞，可见到CNP 分布于细胞内，而未见到对亚细胞结构受损现象。结论 对体外培养细胞的生长繁殖具有促进作用，其作用强度与浓度有关。 关键词: 纳米石墨碳；人肝细胞株；人肝癌细胞株；超微结构 Effects of Graphite Carbon Nanoparticles on Numbers and Ultramicrostructure of Cell Growth in Vitroon Liu Dong-jing , Liu Mei-zhou, Zhang Yang-de, Liu Dong-fe, Pan Yi-feng, Chen Wei, Liu Hui, Zeng Qing-ren, Zhang-hong* Nathonal Hepatobiliary Enteric Surgery Research Center Ministry of Health, Xiangya Hospital, Central South University; No.87, Xiangya Road, Changsha, Hunan 410008, P. R. China. Abstract: Objective To investigate the effects of carbon nanoparticles (CNP) on cell proliferation and ultramicrostructure. Methods: Different concentrations of carbon nanoparticles were added into the HepG2 cells、L02 cells、Hl7702 cells and 3T3 cells to observe the effects of carbon nanoparticles on cell proliferation. The hemacytometry were used to count the number of cells. The cell submicrostructure was observed through transmission electron microscope. Related statistical methods were used to analyze the results. Results: When the medium was added with carbon nanoparticles, the number of L02 cells、Hl7702 cells and 3T3 cells increased, reaching the highest when the concentration of carbon nanoparticles was 7.5μg /ml. Under the transmission electron microscope, it was found that when the concentration of carbon nanoparticles was 7.5μg /ml, the particles could easily enter th