应用代谢通量法分析花生四烯酸的合成过程-高校化学工程学报.pdf

21 2 No.2 Vol.21 2007 4 Journal of Chemical Engineering of Chinese Universities Apr . 2007 1003-9015(2007)02-0316-06 , , , , ( , 210009) (AA) ME-1(Mortierella alpina ME-1), AA 3.28%8.80%6.97% 96 h 0.05%NaNO3 AA 3.95%19.21%39.29% AA 1.3 g⋅L− 1 3.5 g⋅L− 1 AA TQ920.1 Q591.9 A Metabolic Flux Analysis on Arachidonic Acid Fermentation JIN Ming-jie, HUANG He, ZHANG Kun, YAN Jie, GAO Zhen (College of Life Science and Pharmaceutical Engineering, Nanjing University of Technology, Nanjing 210009, China) Abstract: Analysis of flux distributions in metabolic networks has become an important approach for understanding the fermentation characteristics of the process. A model of metabolic flux analysis of arachidonic acid (AA) synthesis in Mortierella alpina ME-1 was established and carbon flux distributions were estimated in different fermentation phases with different concentrations of N-source. During exponential, decelerating and stationary phase, carbon fluxes to AA are 3.28%, 8.80%, 6.97%, respectively, with sufficient N-source broth based on the flux of glucose uptake, and those are increased to 3.95%, 19.21% and 39.29%, respectively, by regulating the shifts of carbon fluxes via fermentation with limited N-source broth and adding 0.05% NaNO3 at 96h. Eventually AA yield increased from 1.3 g⋅L− 1 to 3.5 g⋅L− 1. These results suggest a way to improve AA fermentation, that is, fermentation with limited N-source broth and adding low concentration N-source during stationary phase. Key words: metabolic flux analysis; arachidonic acid; Mortierella alpina ; fermentation; synthesis 1 (Arachidonic AcidAA5,8,11,