毕业设计（论文）《玉米C型雄性不育恢复基因Rf5的标记开发研究》.docVIP

PAGE PAGE 1 玉米C型雄性不育恢复基因Rf5的标记开发研究 （ 农学院农学08-2班） 摘要：玉米是主要的粮食、饲料及工业加工作物，中国是玉米第二大生产国，提高玉米产量、改善玉米品质和增强玉米抗性。实现玉米生产的高产、优质、广适抗病不仅是常规育种的主要目标，也是生物技术研究的重要内容之一。玉米DNA分子标记研究始于1975年．分子标记是继形态标记细胞学标记和生化标记之后发展起来的一种新的较为理想的遗传标记，本文概述了几种主要的分子标记的基本原理和特点，笔者对SSR标记、InDel标记、Intron标记的原理及优、缺点进行了阐述，并运用上述三种标记设计了相应的引物，并发现了一些具有多态性的引物。SSR引物：umc1792、bnlg1885,chr-5.09-1-8、chr-5.09-1-14、chr-5.09-1-52；Intron引物：d-108；Indel引物：H-1、H-56、H-118。并用上述引物上了大量群体，得到了一些数据，作图并进行了玉米C型雄性不育恢复基因Rf5的相关研究。 关键词：玉米：Rf5：标记开发 Corn C male sterility of restoring genes Rf5 mark development research LI Zhong-yun (Agriculture 2008-2 of Agronomy College) Abstract：Corn is the main food, feed and industrial processing plants, China is the second largest producer of corn, improve maize yield, improve quality and enhance corn corn resistance. Realize the corn production of high yield, high quality,Wide suitable resistance , Not only is the main goal of the conventional breeding, biotechnology is also one of the important research topics,The research of Corn DNA molecular markers started in 1975. Molecular marker is the form markers H cells mark and biochemical mark after developed a kind of new and more ideal genetic markers, this paper summarizes several main molecular markers of the basic principle and characteristics, and the advantages and disadvantages of them are compared. The author SSR molecular marker, InDel mark, Intron mark of the principles and advantages and disadvantages were introduced, and apply the above three tag design corresponding primers, and found that some of the diversity of primers. SSR primer: umc1792, bnlg1885, Chr-5.09-1-8, Chr-5.09-1-14, Chr-5.09-1-52; Intron primer: d-108; Indel primer: H-1, H-56, H-118. And a lot of the primer on group, get some data, Mapping and corn C male sterility of restoring genes Rf5 related research Keywords：Corn;Rf5;marker development 遗传标记是基因型的特殊的易于识别的表现形式，它是生物分类学、起源与进化等研究的主要技术指标之一。理想的遗传标记主要应具备以下几条标准：1.具有较强的多态性；2.表现为共显性，因而能鉴别出纯合基因型和杂合基因型；3. 选择中性；传统上一直沿