甘蔗褐条病病原菌分离鉴定及其室内毒力的测定.docxVIP

热带作物学报2015，36(2)：353—357Chinese 热带作物学报2015，36(2)：353—357 Chinese Joumal of TI．0pical Crops 甘蔗褐条病病原菌分离鉴定 及其室内毒力的测定 钱双宏1，一，沈林波2，熊国如2，王俊刚2，冯翠莲z，赵婷婷2，张树珍z+ 1海南大学农学院．海南海口 570228 2裹释蠢辚豸兹辫誓辫囊嚣萎槲二农业部热带作物生物技术重点开放实验室 湃『早J，耳。 m，川¨U1 摘要针对海南省不同植蔗区甘蔗褐条病的典型病斑进行分离培养、单孢纯化、形态学观察、致病性检测以 及结合rDNA—I髑序列分析鉴定病原菌。分离到的5株病原菌。经回接实验表明。病原菌HT-4致病性最强。能 引起与田间病害一致的症状，将菌株H7r_4的rDNA—I髑序列在NCBI上进行BLAST比对。结果表明：菌株HT-4 与离蠕孢属(曰ipoZ砒)相似性达到99％，结合形态学分析确定该菌是甘蔗褐条病的病原菌。室内毒力实验表明： 在7种供试药剂中，50％咪鲜胺锰盐和10％苯醚甲环唑对病原菌抑制效果最好，EC。值分别为4．400 6 m玑和 9．4211 m玑。结果为甘蔗褐条病的防控提供科学参考。 关键词 甘蔗褐条病；离蠕孢属；室内毒力测定；杀菌剂 中图分类号 S435．661 文献标识码 A IsOlation，Identi6cation and Laboratory Toxicity Dete珊iIIation of the Pathogen of Sugarcane Brown Stripe QIAN shuanghongL2，sHEN Linb02，xIoNG Guom2，wANG Jungan92 FENG cuilian2，zHAO Tin昏in矛，zHANG shuzhen2+ 1 Co如矿矿A咖c砒m，日越M‰西em吼删tD地日越na，l 57∞28，吼im 2 s嶝w删Rese帆JIl＆mer’胁舭船矿脚论以口洒c话Me讲以肋据c^删嗍；cAmls／‰，，曰i毗c^加l吲 ￡曲。删吖加脚如面Crops，埘蕊町D，A g，电础啪，舶疏D地月矗，啪57J』0J，蕊f∞ Abstract In tlle study，tlle typical symptomatic tissues of sugarcanes bmwn stripe disease were collected f如m various sugarcane producing of Hainan．The pathogen of sugarcanes brown stripe disease w躯isolated and cultured．Moreover，single spore isolates，morpholo舀cal obsen，ation，patllogenicity test，and combined wit}l molecular biology methods were used to identi母tlle pa山ogen．The inoculation experiment showed tllat tlle strain HT—4 was tlle most vimlent and aU of tlle 5 strains caused symptoms consistent witll mose observed in the fields．rDNA一玎3 of tlle strain HT_4 were submitted NCBI to conduct tlle BLAST analvsis．ne strain HT-4 showed 99％of identity widl曰咖02砒，indicating that，the strajn HT_4 was tlle patlIogen causing sugarc觚e browIl stripe． Laboratory toxicity tests indic砒ed tllat tlle 50％pmchlomz—manganese chloride complex蚰d 10％difenocona∞le had s呐ng suppressing activites against tlle patllogen，winl如Ec50 0f 4．400 6 mg，L鲫d 9．42l 1 mg，Lrespectively． The study could provide valuable reference for t}le prevention柚d cont