穿琥宁及其复方注射液解热作用的比较研究.docVIP

???? 穿琥宁及其复方注射液解热作用的比较研究 ???? ????　提要　通过热反应法测量家兔给药前后直肠温度变化的数据，证明了以穿琥宁为主药，配伍适量异丙嗪，具有药物协同作用的复方穿琥宁制剂的解热作用优于单方制剂。　　关键词　复方穿琥宁注射液　协同作用　解热作用 STUDY ON ANTIPYRETIC EFFECT OF CHUANHUNINGCOMPOUND INJECTION IN COMPARISON WITH OTHERRELATIVE PREPARATIONS Wang Bingnan　He Yingju　Wang Xiang　Long Bo(School Pharmacy, West China University of Medical Sciences　Chengdu 610041)Guo Lingxiao　Zhang Yunwei　Ding Zhiyong　Chen Lin　(Wuliangye Group-Yibin Pharmaceutical Factory　Yibin 644002) 　　ABSTRACT　The antipyretic effects of Chuanhuning compound injections with low and normal doses were studied in this paper compared with Chuanhuning simplex injections with low and normal doses, promethazine simplex and normal saline injections. The results showed that the antipyretic effect of Chuanhuning compound injection with normal dose was stronger than that of any other relative preparations and the antipyretic effect of Chuanhuning compound injection with low dose was stronger than that of Chuanhuning simplex injection with low dose, promethazine simplex injection, normal saline injection and equivalent to that of Chuanhuning simplex injection with normal dose, both statistically and pharmacodynamically, respectively. The reasonable explanation should be atributed to the synergism of drugs.　　Key Words　Chuanhuning compound injection　Synergism　Antipyretic effect 　　穿心莲［Andrographis paniculata(Burm f.)Nees］的有效成分穿心莲内酯与琥珀酸酐在氢氧化钾吡啶溶液中反应，生成脱水穿心莲内酯琥珀酸半酯单钾盐，商品名为穿琥宁(Chuanhuning)。　　穿琥宁对呼吸道感染及婴幼儿肺炎有较好疗效。鉴于市售品仅有单方注射剂，制剂单调，疗效有待改善。为了增强单方制剂解热、镇痛和镇静效果，依据药物剂型设计原理，利用药物间协同作用［1，2］，作者研究、开发了复方穿琥宁注射剂。采用温差均数作为解热实验指标，生理盐水作为对照，评价正常剂量复方穿琥宁(穿琥宁100 mg/kg［4］、异丙嗪2.5 mg/kg［2-3］)、正常剂量单方穿琥宁(100 mg/kg)、低剂量复方穿琥宁(穿琥宁10 mg/kg、异丙嗪2.5 mg/kg)、低剂量单方穿琥宁(10 mg/kg)及单方异丙嗪(2.5 mg/kg)的解热作用。 　　1　药品、试剂、仪器 　　单、复方穿琥宁注射液(五粮液集团-宜宾制药厂)；盐酸异丙嗪注射(上海天丰药厂)；生理盐水(四川简阳制药厂)；伤寒、副伤寒甲、乙三联菌苗(卫生部成都生物制品研究所)。注射器、针头、体温表等。 　　2　实验方法与结果 　　2.1　解热曲线的绘制　　2.1.1　实验动物　大耳白兔，体重(1.6～2.0) kg，雌雄各半(华西医科大学实验动物中心)。选择体温波动不超过0.2℃者供用，随机分组，每组8只，共6组。　　2.1.2　测定家兔正常体温　禁食6 h，每隔1 h测定一次肛温