动态光散射测量血小板质量和细菌.pptVIP

* * * * Dynamic Light Scattering Measuring Platelet Quality and Bacterial ContaminationElisabeth Maurer, Ph.D. Canadian Blood Services, RD VancouverUBC, Department of Pathology and Laboratory MedicineUBC Centre for Blood Research * of 17 The Problem Platelet transfusions used to treat and/or prevent bleeding Number of platelet transfusions produced per year: Canada: 300,000 United States: 2,000,000 Europe: 2,500,000 Platelets are sensitive to stress (e.g., cold damage) Activated platelets do not help patients. Storage at 22oC to prevent cold damage Increased risk for bacterial growth Shortages - addressed by increasing donor recruitment * of 17 Outline The Problem Clinical Situation Solving the problem Novel Approach Data Acquisition DLS-PM score The Invention Technology Background The DLS-PM Accomplishments Clinical Results Summary Other Technology Accuracy, Sensitivity * of 17 No non-invasive in vitro test available Tested inventory cannot be transfused Platelet quality / bacterial testing cannot be mandated Good outcome ? Assumption: good platelets Bad outcome ? Assumption: bad platelets Transfusion outcome serves as retrospective platelet quality indicator. The Problem: Clinical Situation * of 17 The Problem: Summary at least 20% discard rate Additional 10% discarded after testing (invasive technology) ? Unknown quality of transfused platelets $1,000 / transfusion ? Worldwide loss of at least 1 billion dollars per year In summary: Unknown platelet quality is a safety issue Discarded platelet transfusions are a financial burden * of 17 Multi-dimensional quality measurement DLS-PM detects discoid platelets activated platelets microparticles bacteria at the same time Solving the Problem: Novel Approach * of 17 88% Platelets 1179 +/- 173 nm 50-100 nm 1200 nm Solving the Problem: Data Acquisition BACTERIA (if present) 500 +/- 60 nm 500 nm 10% Micropart