双水相萃取在中药活性成分提取分离中的应用进展.pdf

·766 · 中草药 Chinese Traditional and Herbal Drugs 第 46 卷 第 5 期 2015 年 3 月 双水相萃取在中药活性成分提取分离中的应用进展 刘磊磊，李秀娜，赵 帅 吉首大学 林产化工工程湖南省重点实验室，湖南 张家界 427000 摘 要：双水相萃取是一种易于放大、可连续化操作、易于集成技术、绿色环保的新型液/液萃取分离技术，在分离纯化蛋 白质、基因、生物纳米分子、细胞和天然产物等领域有着广泛应用。系统综述近5 年该技术在中药活性成分提取分离中的应 用进展，以期抛砖引玉，推动中药活性成分分离纯化研究的进步和发展。 关键词：双水相萃取；中药；活性成分；提取分离；天然产物 中图分类号：R284.2 文献标志码：A 文章编号：0253 - 2670(2015)05 - 0766 - 08 DOI: 10.7501/j.issn.0253-2670.2015.05.025 Advances in application of aqueous two-phase system in separation and purification of active constituents from Chinese materia medica LIU Lei-lei, LI Xiu-na, ZHAO Shuai Key Laboratory of Hunan Forest Products and Chemical Industry Engineering, Jishou University, Zhangjiajie 427000, China Abstract: Aqueous two-phase system (ATPS) is a new liquid-liquid fractionation technique that has been extensively applied in the separation and purification of natural products including proteins, genetic material, bionanoparticles, cells, and secondary metabolites. Advantages of the technique include scale-up potential, continuous operation, ease of process integration, low toxicity, etc. This paper reviewed the applications in separation and purification of the active constituents from Chinese materia medica (CMM) in recent five years, so as to promote the development of separation of the active constituents in CMM. Key words: aqueous two-phase system; Chinese materia medica; active constituents; extraction and separation; natural products 双水相萃取体系（aqueous two-phase systems ， 表 1 常用的形成双水相体系的单体 ATPS ）一般由2 种聚合物、聚合物和盐、离子液体 Table 1 Monomers commonly used to form ATPS 和盐、小分子亲水有机溶剂和盐组成[1-4] 。当2 种成 形成双水相的成分 组成单体 相物质在水中充分溶解后，在分子间相互排斥力和 类型 空间阻碍作用的影响下，经过一段时间平衡，形成 聚合物 聚乙二醇、葡聚糖、聚丙二醇、聚乙 互不渗透的两相体系。随着研究的深入