脑室内注射组胺受体拮抗剂对神经病理性疼痛大鼠的治疗效果.docVIP

脑室内注射组胺受体拮抗剂对神经病理性疼痛大鼠的治疗效果 黄亮 足立尚登 長櫓巧 新井達 基金项目：日本笹川医学研究者资助项目 作者单位：100730 中国医学科学院 清华大学北京协和医学院 北京协和医院麻醉科（黄亮）；日本愛媛大学附属病院麻酔科（足立尚登，長櫓巧, 新井達） Effect of intracerebroventricular injection of histamine receptor antagonists on mechanical pain threshold of neuropathic pain rat HUANG Liang, NAOTO Adachi, TAKUMI Nagaro , Arai Tatsuru. Department of Anesthesia, Peking Union Medical Hospital, Chinese Academy of Medical Sciences, Beijing 100730 , China [Abstract] Objective To investigate the effect of intracerebroventricular injection of histamine receptor antagonists on mechanical pain threshold of neuropathic pain rat to find out whether there is an antinociceptive effect of histamine receptor antagonists on neuropathic pain. Methods In neuropathic pain rats, the sciatic nerve was partially ligated. Two weeks later, histamine receptor 1 and 2 antagonists—pyrilamine and ranitidine, and saline was injected intracerebroventricularly into neuropathic pain rats and the effect of these medicine on mechanical pain threshold was measured. Results Although there is no signifigant difference in the pain threshold between the saline group and the pyrilamine groups, ranitidine significantly increased the pain threshold compared with the control group, induced antinociception in rats with neuropathic pain. And the efficacy and time duration of the analgesic effect of ranitidine correlate positively with dosage. Conclusion Histamine receptor 2 antagonists can induce antinociception in rats with neuropathic pain. [Key words] Histamine receptor; pyrilamine; ranitidine; neuropathic pain [摘要]目的 探讨脑室内注射组胺受体拮抗剂对神经病理性疼痛大鼠的治疗作用。方法：选择左侧坐骨神经部分结扎致神经病理性疼痛大鼠36只，随机分为对照组：脑室内注射生理盐水；组胺受体1拮抗剂－pyrilamine组（1.5μg和15μg）；组胺受体2的拮抗剂－雷尼替丁组（10μg和100μg），评价注药后痛阈的改变。结果：与对照组相比，雷尼替丁能显著改善疼痛，提高了大鼠对机械性刺激的痛阈（P0.05）。并且随着剂量增加，雷尼替丁对疼痛的治疗效果加强，时间延长。结论：实验证明了神经病理性疼痛时，采用组胺受体2拮抗剂能提高痛阈，可以缓解神经病理性疼痛。 [关键词] 组胺；神经痛；受体；神经递质 在以前的实验中，我们发现神经病理性疼痛大鼠脑内纹状体的组胺代谢较对照组大鼠的代谢发生改变，组胺代谢转换率提高。氯苯氨啶诱导疼痛组大鼠纹状体内组胺水平增高的程度明显大于对照组，且疼痛组大鼠痛阈明显升