在体生物光学成像技术的研究进展.docVIP

第XX卷第X期自动化学报Vol.XX,No.X 200X年X月ACTA AUTOMATICA SINICA Month,200X 在体生物光学成像技术的研究进展 李慧1,2戴汝为2 摘要在体生物发光成像和在体荧光成像是近年来新兴的在体生物光学成像技术,能够无损实时动态监测被标记细胞在活体小动物体内的活动及反应,在肿瘤检测、基因表达、蛋白质分子检测、药物受体定位、药物筛选和药物疗效评价等方面具有很大的应用潜力.本文详细介绍了在体生物发光成像和在体荧光成像的特点、系统及应用,比较了它们的异同,综述了在体生物光学成像技术的基本原理和应用领域,讨论了将其应用于临床的进一步发展方向. 关键词在体生物光学成像,生物发光成像,荧光成像 中图分类号R319 Development of In Vivo Optical Imaging LI Hui1,2DAI Ru-Wei2 Abstract With the emergence of in vivo optical imaging,bioluminescence imaging and?uorescence imaging can be used to non-invasively monitor the activities and response of cells marked with optical signals in real time,which are considered to be promising tools for tumor detection,gene expression pro?ling,protein molecular detection,drug receptor localization,drug screening and therapeutic evaluation.In this paper,the features,imaging systems and applications of in vivo bioluminescence imaging and in vivo?uorescence imaging have been compared and introduced in detail.The basic theories,application?elds and development in future of in vivo optical imaging are especially reviewed. Key words In vivo optical imaging,bioluminescence imaging,?uorescence imaging 随着荧光标记技术和光学成像技术的发展,在体生物光学成像(In vivo optical imaging已经发展为一项崭新的分子、基因表达的分析检测技术,在生命科学、医学研究及药物研发等领域得到广泛应用,主要分为在体生物发光成像(Bioluminescence imaging和在体荧光成像(Fluorescence imaging两种成像方式[1?2].在体生物发光成像采用荧光素酶(Luciferase基因标记细胞或DNA,在体荧光成像则采用荧光报告基团(GFP、RFP等进行标记[3].利用灵敏的光学检测仪器,如电荷耦合摄像机(Charge coupled device camera,CCD camera,观测活体动物体内疾病的发生发展、肿瘤的生长及转移、基因的表达及反应等生物学过程,从而监测活体生物体内的细胞活动和基因行为[4?8]. 收稿日期2007-08-08收修改稿日期2007-11-09 Received August8,2007;in revised form November9,2007 国家自然科学基金北京市优秀人才资助项目(20061D0501600216,中国博士后科学基金(20070410146和中国科学院王宽诚博士后工作奖励基金资助资助 Supported by National Natural Science Foundation of ChinaBeijing Distinguished Specialists Grant (20061D0501600216,China Postdoctoral Science Foundation (20070410146and Chinese Academy of Sciences K.C.Wong Postdoctoral Fellowships 1.首都师范大学教育技术系北京100037 2.中国科学院自动化研究所复杂系统与智能科学重点实验室北京100080 1.D