微流控分析芯片制作.pptxVIP

第九章 微流控分析芯片;Do you know?; Research into miniaturization is primarily driven by the need to reduce costs by reducing the consumption of expensive reagents and by increasing throughput and automation. For example, most are aware of the increasing cost of health care, driven in part by the cost of implementing the latest diagnostic assays. These assays, which are usually performed in microtiter plates that consume hundreds of microliters of reagents, would benefit from the use of microfabricated arrays of nanoliter volume vials. By reducing reagent consumption by a factor of 103–104, these devices could provide dramatic savings for the repetitive assays often performed in diagnostic laboratories.;;尺寸效应（cm→100?m） 尺寸 1/100 分子扩散时间 1/10,000 (1h→0.36s) 体积 1/1,000,000 试剂用量 1t→1g, ml→nl 传热速度 1500oC/s 由此将带来： 方法上的变革 理论上的突破 还有巨大的经济和社会效益;阵列芯片;微流控分析芯片;参考文献 ;目 录;目 录;The Early Days: 1975-1989;;However, the response of the scientific community to this first silicon chip device was virtually none, presumably because of the lack of technological experience (of the separation scientists) to deal with this kind of device. the research work related to miniaturization on silicon focused on the fabrication of components such as micropumps, microvalves, and chemical sensors. ;The Renaissance: 1990-1993;;;Growing to Critical Mass: 1994-1997;分类与特点;目 录;9-2 相关理论 9-2-1 基本概念;液体流动的特点;液体流动的特点;;扩散的数字模拟;不同尺寸通道的数字模拟;;目 录;微结构的形成1．经典的光刻技术;微结构的形成2．模版浇注法（模塑法）;微结构的形成3．模版热压法;微结构的形成4．激光刻蚀法;芯片的封装1．热键合;芯片的封装2．阳极键合;芯片的封装3．室温键合;芯片的封装4．贴合 将聚合物薄片直接覆盖在玻璃或石英板上。 5．压合;表面修饰1．作为色谱固定相;表面修饰2．固定化酶;表面修饰3．固定化生物膜;表面修饰4．亲水或疏水修饰;与外部溶液连接;与质谱仪连接;外加压力驱动;重力驱动;离心力驱动;毛细作用驱动;利用电渗流驱动与控制;利用表面性质控制;单向阀;目 录;9-4 单元操作 9-4-1 样品制备;Sonication ;A microfluidic cartridge to prepare spores for PCR analysis;Extraction ; Concept of sequential ion-sensing system using single microchip ; Schematic illustration of experimental setup and ion pair extraction model ;deter