脱落酸通过影响生长素合成及分布抑制拟南芥主根.pdf

2014 11 22 11 Chinese Journal of Eco-Agriculture, Nov. 2014, 22(11): 1341−1347 DOI: 10.13930/ki.cjea.140240 * 1,2† 1,2† 1 1 1** 袁冰剑 张森磊 曹萌萌 王志娟 李 霞 (1. 050022; 2. 100049) 脱落酸(ABA)在介导植物生长发育及逆境响应中发挥重要功能, 但ABA 抑制根伸长的机制尚不清楚。 本文以拟南芥为材料, 通过研究 ABA 对拟南芥根伸长的影响以及 ABA 受体突变体根发育表型的鉴定, 探讨 ABA 抑制植物主根的机制。研究发现: ABA 能够抑制主根生长及伸长, 并且经典受体PYR1/PRL 介导了ABA 抑 制根伸长的过程; ABA 能够改变细胞周期蛋白CycB1;1::GUS 表达模式, 并影响根中生长素分布和响应。结果表 明, ABA 可能通过影响生长素在根部的分布和剂量, 进而影响根尖分生区细胞分裂, 从而抑制根伸长。 脱落酸 生长素 细胞分裂 主根生长 拟南芥 : Q942.5 : A : 1671-3990(2014)11-1341-07 ABA modulates root growth through regulating auxin in Arabidopsis thaliana 1,2 1,2 1,2 1 1 YUAN Bingjian , ZHANG Senlei , CAO Mengmeng , WANG Zhijuan , LI Xia (1. Center for Agricultural Resources Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Shijiazhuang 050022, China; 2. University of Chinese Academy of Sciences, Beijing 100049, China) Abstract As sessile organism, plants exhibit an amazing developmental plasticity in adapting to changing growth conditions in response to environmental stimuli. As an important organ of plants, roots variously make active changes in the face of different stimuli. It is well known that ABA (abscisic acid) is an important molecule that inhibits root growth. ABA receptor PYR1/PYL mediates ABA signaling transduction by binding with PP2C proteins and activating kinases SnRK2s. However, little is k