汪娜1,1HASSAN1AHMAD,贾永明1薛迪,RAM.pdfVIP

UHPLC-MS 法鉴定大鼠灌服开心散后血中远志糖酯类化合物及其代谢产物1△ 1 1 1 1 1 2 1 汪娜 , HASSAN AHMAD , 贾永明 , 薛迪 , RAM CHAULAGAIN , 黄树明 , 刘学伟 * 1.齐齐哈尔医学院神经药理研究室，黑龙江 齐齐哈尔 161006； 2.黑龙江中医药大学中医药研究院，黑龙江 哈尔滨 150040） 摘要：本研究利用超高效液相色谱-飞行时间质谱联用技术(UHPLC-TOF/MS) ，借助液质 联用工作站Masslynx V4.1 分析软件，结合现有文献及相关数据库，鉴定了开心散60%乙 醇提取物 KXS-60%E ）中20 个远志糖酯类成分；在此基础上，进一步指认并鉴定了灌 服KXS-60%E 后大鼠血中 14 个远志糖酯类化合物及4 个来源于远志糖酯类成分的代谢 产物。以上研究结果表明，远志糖酯类化合物及其代谢产物是KXS-60%E 的主要血中移 行成分，原形及代谢产物可能是开心散防治AD 的潜在药效物质基础。 关键词：开心散；血中移行成分；远志糖酯；代谢产物 Identification of polygala oligosaccharide esters and their metabolites in rat plasma after oral administration of ethanol extract of Kai Xin San by UHPLC-MS 1 1 1 1 1 2 WANG Na , HASSAN AHMAD , JIA Yong-ming , XUE Di , RAM CHAULAGAIN , HUANG Shu-ming , 1 LIU Xue-wei * (1. Department of Neuropharmacology, Research Institute of Medicine and Pharmacy, Qiqihar Medical University, Qiqihar 161006, China; 2. Research Institute of Chinese Medicine, Heilongjiang University of Chinese Medicine, Harbin 150040, China) Abstract: Using a UHPLC-TOF/MS method combined with software of Masslynx V4.1 and database in the literatures, a total of 20 polygala oligosaccharide esters (POEs) were identified in 60% ethanol extract of Kai Xin San (KXS-60%E). Furthermore, 14 POEs as well as 4 POE-metabolites were identified in rat plasma. The present results revealed that POEs and POE-metabolites which were identified in rat plasma, were key components in KXS-60%E and were suggested to be the potential bioactive compounds and direct basis of action of the KXS for treatment of AD. Key words: Kai Xin San; blood transitional component; polygala oligosaccharide ester; 收稿日期：2017-04-17；修回日期：2017-06-30. 基金项目：国家自然科学基金青年基