质粒提取与酶切电泳实验报告.docxVIP

Preparation of Plasmid DNA, Restriction Enzyme Digestion, and Agarose Gel Electrophoresis 2014/10/14-21 1 Intro Objective To learn The characteristics of plasmid DNA The method of plasmid DNA mini-preparation by alkaline lysis and the measurement of DNA concentration by spectrophotometer The characteristics of restriction endonuclease How to use agarose gel electrophoresis to separate DNAs To understand: The principles of purification and quantification of plasmid DNA Principle Plasmid and Vector Plasmid is a small, independently replicating, piece of extrachromosomal cytoplasmic DNA( double