法尼酯X受体上调肝细胞中成纤维细胞生长因子21的表达.docVIP

法尼酯X受体上调肝细胞中成纤维细胞生长因子21的表达 王永超1，刘红2，彭家和1，李良鹏1，董金瑜1，张艳1，江渝1(400038 重庆，第三军医大学基础部生物化学与分子生物学教研室1，400042 重庆，第三军医大学新桥医院血液科2，重庆，400038） [摘要] 目的 研究法尼酯X受体（farnesoid X receptor，FXR）对成纤维细胞生长因子21（fibroblast growth factor,FGF21）表达的影响。方法 用终浓度为0、50、100 μmol/L的CDCA和0、1、5 μmol/L的GW4064分别处理人肝癌细胞株HepG2和人胚胎肝细胞L02细胞后，用逆转录-聚合酶链法（RT-PCR）检测FXR特异性靶基因小异源二聚体配体（small heterodimer parterner,SHP）和FGF21 mRNA的表达变化，再用westen blot法检测FGF21蛋白表达水平的变化。结果结果应再详细一些，重要指标的具体数值应该放到摘要中 用FXR特异性激动剂CDCA和GW4064刺激后，HepG2和L02细胞内SHP mRNA水平均明显升高，表明FXR在两种细胞中被激活，而FGF21 mRNA和蛋白水平均明显升高（P0.05）。结论 激活的FXR可以上调FGF21的表达。 结果应再详细一些，重要指标的具体数值应该放到摘要中 [关键词] 法尼酯X受体（FXR）；成纤维细胞生长因子21(FGF21)；鹅脱氧胆酸（CDCA）；肥胖 [中图法分类号] [文献标志码] A Farnesoid X receptor upregulates expression of fibroblast growth factor 21 in liver cells Wang Yongchao1,Liu Hong2,Peng Jiahe1,Li Liangpeng1,Dong Jinyu1,Zhang Yan1,Jiang Yu1(1Department of Biochemistry and Molecular Biology，College of Basic Medical Sciences，Third Military Medical University，Chongqing，400038，Department of Hematology,Xinqiao Hospital,Third Millitary Medical University,Chongqing,400037,China) [Abstract] Objectives To investigate the effect of farnesoid X receptor (FXR)on the expression of fibroblast growth factor21(FGF21) Methods Treated human hepatoblastoma HepG2 cells and hepatocyte L02 cells with FXR agonist chenodesoxycholic acid(CDCA) at 0、50 and 100μmol/L or GW4064 at 0、1 and 5μmol/L respectively.The FXR specific target gene small heterodimer partner(SHP) and the FGF21 mRNA level were dectected by reverse transcription-polymerase chain reaction(RT-PCR),and the FGF21 protein level was determined by western blotting. Results 同中文摘要修改After treated with CDCA and GW4064 SHP mRNA was increased in HepG2 an L02 cells,it demenstrated that FXR was functional in those cells.The mRNA and protein levels of FGF21 were also increased in those cells(P0.05) Conclusion Farnesoid X receptor upregulated the expression of FGF21 in hepatoctyes. 同中文摘要修改 [Key words] Farnesoid X receptor（