碳酸酐酶微生物诱导矿化修复混凝土早期裂缝.pdfVIP

中国科技论文在线 碳酸酐酶微生物诱导矿化修复混凝土早期 裂缝# 任立夫，钱春香** 5 （东南大学材料科学与工程学院，绿色建材技术研究所，南京 211189） 摘要：本文选用一类嗜碱芽孢杆菌，其代谢产物碳酸酐酶可促进二氧化碳可逆水合反应，通 过试验证明，此菌具有修复混凝土裂缝的能力。试验中通过对纯化菌株的生长曲线测定，确 定其最佳培养时间为24-28h ，最适pH 为8。随着pH 升高，碳酸酐酶菌生长能力和酶化活 10 力下降，但pH 为12-13 时仍存在活菌，并且能够维持一定酶化活力，其对混凝土环境具有 一定的耐受性。在溶液环境中，通过试验证明了碳酸酐酶菌具有加速碳酸钙沉积的能力，进 一步在混凝土裂缝自修复试验中，验证了提供充足钙离子条件下，碳酸酐酶微生物诱导矿化 修复混凝土早期裂缝具有可行性。 关键词：材料学；碳酸酐酶；混凝土；裂缝；修复 15 中图分类号：Q939.99 Restoration of concrete cracks in early age by carbonic anhydrase microbial-induced mineralization REN Lifu, QIAN Chunxiang 20 (College of Materials Science and Engineering, Research Institute of Green Construction Materials,Southeast University,Nanjing 211189) Abstract : In this paper, a class of alkalophilic Bacillus was used. Its metabolites carbonic anhydrase can promote CO2 reversible hydration reaction. The research showed that the bacteria had the ability to repair concrete cracks. Test of growth curve by cultivation experiments 25 determined that the optimal incubation time was 24-28h and the optimal pH was 8. As the pH increased, the growth capacity of bacteria and the activity of carbonic anhydrase decreased. There were still viable bacteria which could maintain a certain enzymatic activity when the pH increased to 12-13. It proved that the alkalophilic Bacillus was compatible with concrete. The bacteria had the ability to accelerate the deposition of calcium carbonate through experiments in solution 30 environment. Further tests verified the bacteria had the ability to repair concrete cracks in early