流式细胞(FACS)多色分析报告.pptVIP

23-13536-00 Rev. 01 Analysis 23-13536-00 Rev. 01 23-13536-00 Rev. 01 Multicolor Analysis 23-13536-00 Rev. 01 * Multicolor Analysis Strategies Plot types Hierarchical gating Population hierarchies Back gating 23-13536-00 Rev. 01 * Use Different Plot Types Histograms—using histograms can help discriminate between two populations. Dot Plot: hard to discriminate populations Dot Plot: displaying fewer events improves discrimination Histogram: population boundary is evident 23-13536-00 Rev. 01 * Use Different Plot Types Contour Plots—using contour plots can: Help locate population means. Indicate a need for biexponential scaling. Dot Plot: hard to see populations bunched on axis Contour Plot: shows that biexponential display might be needed Biexponential Contour Plot: gives best display of populations 23-13536-00 Rev. 01 * Hierarchical Gating 23-13536-00 Rev. 01 * Hierarchical Gating What went wrong here? Correct Hierarchy 23-13536-00 Rev. 01 * Back Gating Researcher wants to discriminate Tregs based on CD25 and CD127 expression. Need to ensure a gate includes the entire FOXP3+ population. 23-13536-00 Rev. 01 * Additional Analysis Strategies Biexponential scaling Events to display Hinged and offset quadrant gates Derived (boolean) gates Snap-to gates Statistics Analysis templates 23-13536-00 Rev. 01 * ICS Data Analysis Results Information that can be retrieved: Number of collected events, number of live CD3+ cells % of main populations: %CD3+, CD4+, CD8+ % of na?ve (CD45RA+) and memory (CD45RA–) CD4+ and CD8+ cells % CD25+CD4+ cells (Tregs) For the cytokines: % of CD4+ and CD8+making 1 or 2 cytokines Analysis Exercise 23-13536-00 Rev. 01 * ICS Panel Analysis 谢谢观看！ 2020 * We are now going to talk about tools and strategies useful when it comes to data analysis. * 1st bullet; Every analysis software will give you an option to look at the data using different plot types. People have their own preferences but we will discuss the advantages of some plot types ove