贺福初院士 人类肝脏蛋白质组计划的实施与毒理学发展的机遇解析.ppt

Progress ? Evaluation of technologies ? Bioinformatics set-up ? Sampling and Preparation ? Preview with human fetal liver ? Primary analysis of French liver tissues It is a giant ambitious objective and gives a great challenge to embryonic proteomics It is necessary to carry out a preview before the formal launching of this project Science 302:1316, Nov. 21, 2003 Nature 425: 441, Oct. 2, 2003 Average Throughput of the Platform ? 2DE System 12 Gel / 3 Days ? Auto-Spot Digest System 1152 spots / Day ? MS and MS/MS System 500-1000 Spots / Day The flowchart of CCPIT for the protein expression profile of human fetal liver The area scales before and after isoform processing in group and protein levels respectively. In protein level, the red circularity areas represent the number of confirmed proteins and the yellow annulus areas represent the number of possible proteins. Non-iso groups 19% Extensively confirmed 81% Groups 41% Confirmed 59% Non-isoforms 43% Isoforms 57% c o n t r i b u t e 5 4 5 a d d i t i o n a l p r o t e i n s a n d e l i m i n a t e 7 6 3 g r o u p s c o n t r i b u t e 5 4 5 a d d i t i o n a l p r o t e i n s a n d e l i m i n a t e 1 3 3 5 p o s s i b l e p r o t e i n s I n P r o t e i n L e v e l I n G r o u p L e v e l c o n f i r m e d : 1 9 5 0 p o s s i b l e : 2 5 9 3 e x t e n s i v e l y c o n f i r m e d : 2 4 9 5 n o n - i s o f