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protocol
Methods for isolation of marine-derived endophytic
fungi and their bioactive secondary products
Julia Kjer, Abdessamad Debbab, Amal H Aly Peter Proksch
Institut für Pharmazeutische Biologie und Biotechnologie, Heinrich-Heine-Universität Düsseldorf, Düsseldorf, Germany. Correspondence should be addressed to
P.P. (proksch@uni-duesseldorf.de).
Published online 18 February 2010; doi:10.1038/nprot.2009.233
s Marine-derived fungi have been shown in recent years to produce a plethora of new bioactive secondary metabolites, some
l
o of them featuring new carbon frameworks hitherto unprecedented in nature. these compounds are of interest as new lead
c
o
t structures for medicine as well as for plant protection. the aim of this protocol is to give a detailed description of methods
o
r useful for the isolation and cultivation of fungi associated with various marine organisms (sponges, algae and mangrove
p
e
r plants) for the extraction, characterization and structure elucidation of biologically active secondary metabolites produced
u
t
a by these marine-derived endophytic fungi, and for the preliminary evaluation of their pharmacological properties based on
n
/ rapid ‘in house’ screening systems. some results exemplifying the positive outcomes of the protocol are given at the end.
m
o From sampling in marine environment to completion of the structure elucidation and bioactivity screening, a period of at
c
.
e
r least 3 months has to be scheduled.
u
t
a
n
.
w
w IntroDuctIon
w
/
/ Terrestrial fungi have long been known as a rich source of bio- micro-organisms including bacteria, cyanobacteria, microalgae
:
p
t
t logically active se
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