自动核酸提取仪原理PCR新参考幻灯片.pptVIP

全自动核酸提取仪基本原理 ? 1 磁笔式 ? 国外： Chemagen ? 国内：厦门百维信，厦门欧达科，博日 ? 2 吸管式 ? 国外： Roche ， Qiagen ，生物梅里埃 1 Chemagen 核酸提取仪原理示意图 2 Chemagen 主要产品 ? chemagic Prepito ? chemagic MSM I 3 chemagic Prepito 4 chemagic Prepito ? 1 – 12 samples per run ? integrated buffer dispensing ? revolutionary resuspension technology ? standard plastic devices ? chemagic Kits and protocols for a huge variety of sample materials ? quantitative yield, highest purity ? sample sizes up to 600 μ l ? bar code reading available 5 chemagic MSM I 6 7 chemagic MSM I ? Sample volumes from 1 μ l to 10 ml processable ? High speed, 15 min. for 96 low volume blood samples ? Low to high throughput (4,000 samples/d) ? 96 rod head for parallel separating and resuspending in 96 well plates ? 12 rod head for parallel separating and resuspending in 15 ml or 50 ml Falcon tubes 8 Fig. 1 Schematic illustration of the chemagic Magnetic Separation Module I for semi-automated extraction of viral nucleic acids from up to 12 minipools in one batch. (a) Separation head containing 12 metal rods. (b) Plastic disposables. (c) Tubes containing samples. (d) and (e) Tubes containing wash buffers. (f) Tubes containing elution buffer. 9 10 其他生产商产品 ? 厦门百维信 Lab-Aid 820 ? 厦门欧达科 MP-120 ? 博日 Gene-pure 11 12 Gene-pure 工作示意图 13 Qiagen BioRobot M96 Workstation BioRobot M48 workstation BioRobot EZ1 Roche MagNA Pure LC 1.0 ， 2.0 14 BioRobot M48 Workstation 15