基因克隆关键技术.docVIP

基因克隆技术 摘要：基因克隆技术是分子生物学关键技术，其目标是取得某一基因或DNA片段大量拷贝，用于深入分析基因结构和功效，并可达成人为改造细胞和物种遗传性状目标。本论文关键从以下多个方面来介绍基因克隆技术：目标基因取得 、目标基因和载体连接、重组分子扩增和判定。 关键词：目标基因；限制性内切酶；克隆；重组分子 ABSTRACT：Gene cloning technology is the core of molecular biology technology, its purpose is obtain a gene or DNA fragments of the copy, used for in-depth analysis the structure and function of genes, and may achieve human cells and the transformation of the species genetics purpose.This thesis mainly from the following several aspects to introduce gene cloning technology: the purpose of the gene for the purpose, genes and carrier, restruc