甘蓝型油菜核糖体大亚基BnaRPLS34基因调控自交不亲和反应的功能研究.pdfVIP

甘蓝型油菜核糖体大亚基基因的全基因组分析与BnaRPLS34 基因调控自交不亲和反应的功能研究 Contents 摘 要I ABSTRACT III LIST OF ABBREVIATIONS V I. LITERATURE REVIEW 1 1.1 INTRODUCTION 1 1.2 OVERVIEW OF HERMAPHRODITIC ANGIOSPERMS SELF-INCOMPATIBILITY SYSTEM 1 1.3 MOLECULAR MECHANISM OF BRASSICACEAE SELF-INCOMPATIBILITY 4 1.6 CONCISE MODEL OF SI SIGNALLING CASCADE IN BRASSICA 10 1.7 RIBOSOMES 10 1.7.1 Eukaryotic ribosome structure and function 11 1.7.2 Compartmentalization of ribosome function and assembly 11 1.7.3 Cytoplasmic ribosomal protein gene family 12 1.8 AUTHENTIC EXTRA-RIBOSOMAL ACTIVITIES OF RIBOSOMAL PROTEINS 13 1.8.1 Ribosomal protein L7 evolved to non-ribosomal protein 13 1.8.2 Signal transduction activity of RACK1 protein far from ribosomes 13 1.8.3 L13a is involved in extra-ribosomal activity 14 1.8.4 Translocation of ribosomal protein S3 from cytoplasm to nucleus 14 1.8.5 Ribosomal protein L10 is involved in anti-viral defense system 15 1.9 RESEARCH PURPOSES AND SIGNIFICANCE 16 2. MATERIALS AND METHODS 18 2.1 CHARACTERIZATION OF RIBOSOMAL PROTEIN LARGER SUBUNIT PROTEINS IN BRASSICA NAPUS 18 2.2 PHYLOGENETIC AND BIO INFORMATIC ANALYSIS OF GENE STRUCTURE AND CONSERVED DOMAINS AND MOTIFS IN BRASSICA NAPUS 18 2.3 PLANT MATERIAL, GROWTH CONDITION AND POLLINATION BIOASSAY 19 2.4 RNA EXTRACTION 20 2.5 DIFFERENTIAL EXPRESSION AND EXPRESSION AFTER POLLINATION BIOASSAY 20 2.6 TRANSGENE CONSTRUCTS AND PLANT TRANSFORMATION 21 2.7 STIGMA COLLECTION AND ANILINE BLUE ASSAY 22 2.7.1 Self-incompatibility Assay 22 2.7.2 Mutant screening and validation of genome editing 23 2.8 Tissue specific expression analysis 23 3. RESULTS 24 3.1 GENOME WIDE IDENTIFICATION OF SI SPECIFIC B. NAPUS RPLS GENES 24 3.2 PROTEINS PROPERTIES AND SEQUENCE ANALYSIS OF BNARPLS 2