胆汁致胃黏膜损伤中瘦素、环氧合酶2及p27的表达(精).docxVIP

胆汁致胃黏膜损伤中瘦素、环氧合酶 -2 及 p27 的表达 [ 11-01-09 15:45:00 ]  作者：宋银雪，龚 均  编辑： studa20 【摘要】 目的 通过大鼠实验模型观察瘦素 (leptin) 、环氧合酶 - 2(COX-2)及 p27 在胆汁所致胃黏膜损伤中的作用。方法 SD 大鼠分成 4 组：十 二指肠胃反流 (duodenogastric reflux, DGR) 组、 DGR+胆管结扎 (bile duct ligation, BDL) 组、胆管结扎组 (BDL)、假手术组 (control, C) 组。术后 12 周 处死大鼠，观察胃黏膜损害及病理组织学改变，计算 DIXON积分；免疫组化 (SP) 法检测各组大鼠胃黏膜瘦素、 COX-2及 p27 表达。结果 DIXON 积分 DGR组、DGR+BDL组较 BDL组及假手术组积分均升高 (P0.05) ，DGR组较 DGR+BDL组DIXON积分升高 (P0.05) ； DGR组、 DGR+BDL组与 BDL组及假手术组比较大鼠胃黏膜瘦素、 COX-2高表达 (P0.05) ，p27 低表达 (P0.05) ， DGR组比 DGR+BDL组高表达尤为明显 (P0.05) 。DIXON积分与瘦素、 COX-2表达呈正相关，与 p27 表达呈负相关。结论 胆汁反流致胃黏膜损伤过程中，瘦素、 COX-2及 p27 蛋白与病变的修复过程有关。 【关键词】  胆汁 胃黏膜 瘦素  COX-2 p27 ABSTRACT: Objective To explore the role of leptin, COX-2 and p27 in bile-induced gastric mucosal injury in rats. Methods  SD rats were divided into 4 groups: DGR (duodenogastric reflux) group, DGR+BDL (ligation of bile duct) group, BDL group and control group. The pathological changes of gastric mucosa and tight junction were observed, DIXON scores were warked out after 3 months. Immunohistochemistry was used to detect the expression of leptin, COX-2 and p27 in gastric mucosa with different lesions. Results The DIXON scores in DGR and DGR+BDL group were higher than those in BDL group and control group (P0.05). In addition, DIXON scores were higher in DGR group than in DGR+BDL group (P0.05). The expressions of leptin and COX-2 increased in DGR group and DGR+BDL group than in BDL group and control group (P0.05), while p27 decreasd (P0.05). DGR group was more notable than DGR+BDL group (P0.05). The expression of leptin and COX-2 was positively correlated with DIXON integral, but negatively related to p27. Conclusion Leptin, COX-2 and p27 are all concerned with renovating pathological changes in bile-induced gastric mucosal injury in rats. KEY WORDS: bile; gastric mucosal; leptin; COX-2; p27 近年研究发现反流至胃内的胆汁、胰液和肠液等十二指肠内容物可造成胃黏膜细胞损伤［ 1］。瘦素、 COX-2和 p27 这三种因子均可通过调节细胞周期来 调节胃黏膜细胞增殖及凋亡。瘦素在细胞内能与许多信号分子相互作用，激活JAK/STAT、MAPK、磷酸肌醇和脂类代谢等信号通路，参与多