丝裂素激活蛋白激酶在良性前列腺增生中的表达及临床意义.docVIP

ERK在良性前列腺增生)I?的表达及意义 张立春⑴，李凯⑺,王平⑺ 中国医科大学附属盛京医院急诊科；中国医科大学附属第四医院泌尿外科，辽宁沈阳U0003 【摘要】 日的 对比研究ERK在增生与正常前列腺组织中的表达，探讨良性前列腺增生发病机制。方 法 采用Western Biol及免疫组织化学方法，检测增生及正常前列腺组织中ERK的表达情况。结果 与 正常前列腺组织相比，增生组织ERK的平均灰度值white?bbck,0?255和阳性细胞百分率明显升高ERK 染色主要位于细胞核和细胞质内，在増生组织上皮细胞及基质细胞的染色阳性率均高于正常询列腺组织。 结论 ERK在良性前列腺增生组织中过表达，从而导致细胞増生指数増加促进疾病的发生和发展。 【关键词】ERK信号传导;良性前列腺增生泼病机制 Expression of ERK in Benign Proslatic Hyperplasia and its Clinical Signillcance ZHANGLichun , LI Kai ,WANG Ping (1 )The Affiliated SHENGJING Hospital; (2.) Department of Urinary Surgery ,Fourth Affiliated Hospital ,China Medical University ,Liaoning Shenyang 110001 China [Abstract ] Objective To investigate the expression of erk in benign prostalic hyperplasia (BPH) and normal prostatic tissue and explore the molecular biology mechanism of pathogenesis of BPH. Melhods To detect the expression of extracellular signal regulated kinase (ERK) in 64 cases of BPH and 16 cases of normal prostates with western blot analysis and immunohis to chemical techniques? Results The average gray value of ERK and percentage of ERK positive cells were higher in BPH compared with normal prostates P v0? 01 ? The immunostaining product of ERK was mainly located in nuclei and cytoplasm, and the positive staining rates of epithelial cells and stromal cells in BPH were higher than that in normal proslate prominent Conclusion The overexpression of ERK leading to the increase of proliterative index of cells leading to the decrease of apoptosis maybe involved in the development of BPH? 【Key words] MAPK; signal transduction; benign prostatic hyperplasis BPH ;pathogenesis MAPK是细胞内信号传递途径的共同通道和汇聚点rLo在Weinberg等曾报 道，有丝分裂原通过激活MAPKs促使细胞增生⑵。本研究通过对比检测良性增 生及正常前列腺细胞屮ERK的表达，探讨BPH发病的分子机制。 1材料与方法 111标本来源和主要试剂 医大一院2003年6月 ?2004年6月经手术切除的增生前列腺组织64例，年 龄57?73岁,标本均由耻骨上前列腺摘除术获得。从厂检屮获取的正常前列腺组 织12例作为对照，年龄24?35岁。所有标本均经两位资深病理医师确认，且 资料完整。切下的组织标本立即用预冷的PBS冲洗后，30 min内置于液氮屮， 以后送于?70°C的冰箱屮保存待检。Western杂交ERK [抗及II抗为promega 公司产詁，购自北京原平皓生物公司;免疫组化SABC试剂盒及DAB显色剂为 武汉博士德生物公司产品。 112实验方法 Weste