胰岛素样生长因子及其受体在无功能性垂体腺瘤的表达及意义.docVIP

IGF-1和IGF-1R在无功能性垂体腺瘤中的表达及意义 王志明|杨刚|蒲九君|孙彦龙|周辉|覃凯|阮伦亮| 1（ 400016重庆 重庆医科大学附属第一医院神经外科） 【摘要】 目的 探讨胰岛素样生长因子1（TGF-1）和胰岛素样生长因子1型 受体（IGF-1R）与无功能性垂体腺瘤侵袭性和复发性的关系。方法60例无功能 性垂体腺瘤分复发性侵袭性组（16例）和非复发性侵袭性组（24例），侵袭性非复 发性组（24例）（两者最好一致）和非侵袭性非复发性组（20例）。采用qRT?PCR 检测IGF-1和IGF-1R的mRNA表达水平，采用免疫组织化学法和Western blot 法检测IGF?1和TGF-1R的蛋白表达水平。结果IGF-1和IGF-1R的mRNA和 蛋白表达水平，复发性侵袭性组高于非复发性侵袭性组（p0.05）,侵袭性非复 发性组高于非侵袭性非复发组（p0.05）o相关性分析IGF?1和IGF-1R正相关（「 =0.85, p0.05）o结论IGF-1和IGF-1R在侵袭性和复发性无功能性垂体腺瘤中 表达上调，两者协调作用，与无功能性垂体腺瘤的侵袭性和复发性恶性行为的发 生发展可能相关。 【关键词】IGF?1 IGF-1R无功能性垂体腺瘤 侵袭性 复发性 【中国分类号】R651【文献标识码】A Expression of insulin-like growth factor 1 and insulin-like growth factor 1 receptor in human non-functioning pituitary adenomas: correlation with invasiveness and recurrence WANG Zhi Ming, YANG Gang, PU Jiu Jun, SUN Yan Long, ZHOU Hui, QIN Kai, RUAN Lun Liang Department of Neurosurgery, The First Affiliated Hospital, Chongqing Medical University, NO. 1 Youyi Road Yuanjiagang, Chongqing 400016, China. Tel： 023作者简介：王志明，硕士研究生，研究方向：垂体腺瘤侵袭性 通讯作者：杨刚，电话：023 — E-mail： yg angcq@ 163.com. [Abstract] Objective To investigate the expression levels of insulin-like growth factor l(IGF-l) and insulin-like growth factor 1 receptor(IGF-lR) in human non-functioning pituitary adenomas and their relationships with tumor invasion and recurrence in these cases. Methods Sixty specimens of non-functioning pituitary adenomas were classified into invasive non-recurrent groups and non-invasive non-rccurrcnt groups, recurrent invasive groups and non-rccurrcnt invasive groups. The protein and mRNA expression levels of IGF-land IGF-1R were detected using immunohistochemistry assay, western blot assay and quantitative real-time polymerase chain reaction (qRT-PCR), respectively. Results The mRNA and protein expression levels of IGF-1 and IGF-1R were significantly higher in invasive non-rccurrcnt group than in non-invasivc non-rccurrcnt group (p0.05) ? The mRNA and protein expression levels of levels of IGF-land IGF-1R were sig