细胞信号通路ppt细胞信号通路大全上下细胞信号通路ppt ras_pathway.pptVIP

R T K R T K Ras Pathway SHC GDP GTP CD-GEGII GAP GTP Elk1 c-Fos ATF2 c-Jun Actin Cytoskeleton P P P P P P Stress Fibers and Focal Adhesions GeneExpression PLDPathway PMA Growth Factors Increased T Cell Adhesion Integrins β1 β2 β2 β1 β1 SOS p120- GAP p190-B Rho PI3K PLC-ε Rap1A PLD RalBP1 PAKs ERKs ERKs JNKK JNK JNK MEKK1 CDC42 Rac MEKs Raf RalGDS Ral GRB2 TCR Antigen Lck GEF Ras Ras 2009 ProteinL C * Review: Ras is a membrane-associated guanine nucleotide-binding protein that is normally activated in response to the binding of extracellular signals, such as growth factors, RTKs (Receptor Tyrosine Kinases), TCR (T-Cell Receptors) and PMA (Phorbol-12 Myristate-13 Acetate). Ras signaling affects many cellular functions, which includes cell proliferation, apoptosis, migration, fate specification, and differentiation. Ras acts as a binary signal switch cycling between ON and OFF states, which are characterized in terms of a small molecule, a guanine nucleotide, bound to the protein. In the resting cell, Ras is tightly bound to GDP (Guanosine Diphosphate), which is exchanged for GTP (Guanosine Triphosphate) upon binding of extracellular stimuli to cell membrane receptors. In the GTP-bound form, Ras interacts specifically with so-called effector proteins, thereby initiating cascades of protein-protein interactions that may finally lead to cell proliferation. To return to the inactive OFF state, Ras cleaves off the terminal phosphate moiety, the Gamma-Phosphate, of GTP in an enzymatic process, the intrinsic GTPase reaction. The remaining GDP-bound Ras is no longer able to interact with effectors, it is switched OFF. Ras is thought to activate a number of signaling pathways, including the Raf/MEK/ERK (Extracellular Signal-Regulated Kinases) pathway, the MEKK/SEK/JNK (Jun N-terminal Kinases) pathway, a PI3K (Phosphatidylinositol 3-Kinase)/Akt/NF-KappaB (Nuclear Factor-Kappa B) pathway, a p120-GAP/p190-B/Rac/NF-KappaB pathway, and a Raf/MEKK1/IKK (I-KappaB Kinase)/I-