甜椒无菌苗培养条件的优化.docxVIP

甜椒无菌苗培养条件的优化 Using Qiemen sweet pepper as materials ， the disinfection effects of different disinfectant agents ， disinfection times and contents on seeds before culture were compared， and the effects of mediumformula ，pH value and growth time on growth of aseptic seedling were investigated. The results showed that the optimum disinfection method and culture conditions for aseptic sweet pepper seedling were soaking seeds in aseptic water of 55 °C for 15 minutes , then staying at room temperature for 4?5 hours , disinfecting with 10%NaCIOfor 30 minutes , washing 3 times with aseptic water and