人体肠道中氯霉素同化菌的分离鉴定与特性研究.docxVIP

Isolation^ identification and characterization of humanintestinal bacteria with the ability to utilize chloramphenicolas the sole source of carbon and energy Zhao Xim, Tian Fengweii, Wang gangi, Liu Xiaomingi, Zhang Qiuxiangi,Zhang Haoi, Chen Wei2 (1. School of Food Science and Technology, Jiangnan University, JiangSu WuXin 214122; 2. State Key Laboratory of Food Science and Technology, Jiangnan University, JiangSu WuXin 214122) Abstract: Five aerobic intestinal bacterial strains that utilized chloramphenicol (CAP) as sole carbon and energy source were isolated from feeal samples collected from healthy volunteers. Based on their morphology, physiological characters, and 16S rDNA sequence analysis, four of the five strains were identified as Klebsiella pneumonia and one as Escherichia fergusonii? The degradation rate of strain I-10-CHL (E? fergusonii) varied with the initial concentration of CAP and the maximum specific substrate removal rate was observed at 25 pg CAP/ml. The pH value also had an effect on the degradation rate of CAP and bacterial growth? A pH of 6.5 was optimal for CAP degradation and growth of strain I-10-CHL (E. fergusonii). In mixed substrate batch cultivations, where CAP was one of the components, glucose, acetamide and CAP were simultaneously utilized. The presence of glucose and acetamide increased the growth and substrate degradation rates of CAP. These results indicate that CAP degrading enzymes are inducible in nature. During incubation with E. fergusonii cells, reduction intennediates (1-p-nitrophcnyl -2-aniino-l, 3-propancdiol) were observed. The products of CAP metabolism by species other than E? fergusonii have not yet been characterized? The strains reducing the antibiotic were chloramphenicol susceptible, indicating that the pathway does not appear to mediate chloramphenicol resistance? The role of this CAP reduction pathway in the physiology of Klebsiella pneumonia and E? fergusonii is unknown. Further understanding of the E? ferg