鲁米诺化学发光分析在药物分析中探究.docxVIP

鲁米诺化学发光剖析在药物剖析中研究 鲁米诺化学发光剖析在药物剖析中研究 PAGE / NUMPAGES 鲁米诺化学发光剖析在药物剖析中研究 鲁米诺化学发光剖析在药物剖析中的研究 化学教育 2000 级3班 梁斐 指导老师：张成孝 教授 纲要 : 化学发光剖析法是依据化学反响产生的光辐射的强度来确立物质含量 的剖析方法。该剖析方法拥有敏捷度高、线性范围宽、仪器简单、剖析速度快、简单实现自动化等长处，遇到人们的宽泛关注。本论文利用鲁米诺系统流动注射化学发光法，成立了双嘧达莫和异烟肼的化学发光剖析法。 我们在实验中发现，将含氮杂环的嘧啶类合成药物双嘧达莫溶液注入鲁米诺 与铁氰化钾化学发光反响结束后的混淆溶液中可产生强的化学发光信号。依据此实验现象并联合流动注射剖析技术，成立了测定双嘧达莫的化学发光剖析法。在 优化的实验条件下，化学发光剖析信号与双嘧达莫的浓度在1×10-10 ~ 4×10-7g/mL 之间呈优秀的线性关系。 依据 IUPAC 规定，计算得该方法的检出限为 6×10-11g/mL 。 对 1×10-8g/mL 的双嘧达莫进行 7 次平行丈量，相对标准偏差为 1.1%。该方法用于双嘧达莫片剂中双嘧达莫含量的测定，并与药典中标准方法进行比较，没有明显 性差别。 实验发现将异烟肼溶液注入鲁米诺与高锰酸钾化学发光反响结束后的混淆溶液中可产生强的化学发光信号。依据此实验现象并联合流动注射剖析技术，成立了测定异烟肼的化学发光剖析法。在优化的实验条件下，化学发光剖析信号与异 烟肼的浓度在 5×10-8 ~ 1×10-4g/mL 之间呈优秀的线性关系。依据 IUPAC 规定，计算得该方法的检出限为 1×10-8g/mL 。对 1×10-6g/mL 的异烟肼进行 7 次平行丈量，相对标准偏差为 1.1%。该方法用于异烟肼片剂中异烟肼含量的测定，结果令人满 意。 重点词 ：流动注射剖析 化学发光反响 鲁米诺 双嘧达莫 异烟肼 1 The analytical application of luminol chemiluminescence in Pharmaceutical assay Liang Fei ZhangChengxiao professor Abstract：Chemiluminescence (CL) analysis promises high sensitivity with simple instrumentation (no monochromator required) and rapidly in signal detection (normally 0.1-10s). When coupled with flow injection analysis (FIA), CL-based FIA methods provide cheap, rapid, simple and reproducible means of detection and therefore, it has been extensively used in many fields, such as biological, clinical, environmental. When dipyridamole(DIP) was injected into the reaction mixture after the finish of the reaction between luminol and K 3Fe(CN)6, a new CL reaction was initiated to produce strong CL emission. Based on this phenomenon, a novel CL system for DIP combined with flow injection analysis was presented in this paper. The CL intensity responded linearly to the concentration of DIP in the range of 1 ×10-10 ~ 4×10-7 g/mL with a detection limit of 6 ×10-11g/mL. The relative standard deviation was 1.1% for 1 ×10-8 g/mL DIP solution( n=7). The method has been applied to determine DIP in tablets and the results are in agreement with traditional method usin