吡啶壳聚糖抗氧化性能初步研究.docVIP

PAGE PAGE 19 吡啶壳聚糖抗氧化性能初步研究 摘要：壳聚糖具有良好的抗氧化活性，在食品、医药、生物医学、水处理、化妆品等多个领域内都具有广泛应用。壳聚糖经吡啶化得到吡啶壳聚糖，本试验采用DPPH自由基清除法、铁氰化钾还原法、β-胡萝卜素亚油酸体系法三种方法，以水溶性壳聚糖为对照，依次比较吡啶壳聚糖的抗氧化性能，初步讨论了各自的抗氧化机制。结果显示：吡啶壳聚糖的抗氧化性明显优于水溶性壳聚糖，其中DPPH自由基清除法显示高于37.6%；铁氰化钾还原法显示高于52.1%；β-胡萝卜素亚油酸体系法显示高于40.5%。对壳聚糖进行吡啶化可拓宽壳聚糖的抗氧化应用领域，具有一定的应用价值。 关键词：吡啶壳聚糖；抗氧化性；DPPH；β-胡萝卜素；铁氰化钾 EVALUATION OF PYRIDINE CHITOSAN’S ANTIOXIDA ACTIVITY Abstract:Chitosan owns good inoxidizability. And it is widely used in the fields of food, pharmaceutical, biomedical, water treatment, cosmetics, etc. Pyridine chitosan is compounded by the reaction of pyridine and chitosan. DPPH free radical scavenging assay, potassium ferricyanide reduction method and β-Carotene-linoleic acid activity were used to evaluste the antioxidant acticity of pyridine chitosan. Water soluble chitosan was the control group. And the fuctional mechanism of antioxidation were also discussed in this research. The research showed that the inoxidizability of pyridine chitosan is batter than water soluble chitosan, which DPPH free radical scavenging assay showed pyridine chitosan is 37.6% higher than water soluble chitosan; potassium ferricyanide reduction method showed 52.1%; β-Carotene-linoleic acid activity showed 40.5%. In order to broaden the applications of chitosan, pyridine chitosan deserves to be reserved deeply. And this will bring greatly utility value. Keywords: pyridine chitosan; inoxidizability; DPPH; β-Carotene; potassium ferricyanide 第1章引言 1.1 壳聚糖的特性及其应用 1.1.1 壳聚糖的特性 1.1.1.1 壳聚糖的结构性质： 壳聚糖是一种由甲壳素在碱性条件下脱乙酰化所得的天然生物高分子聚合物，其学名为β-(1,4)-2-氨基-2-脱氨-D-葡萄糖，又被称为可溶性甲壳素、甲壳胺、壳多糖。通常甲壳素脱乙酰度达到60%，并且能溶解于稀酸的脱乙酰基产物即为壳聚糖， 图1.1 壳聚糖的结构式（n为聚合度） Fig 1.1 structural formula of chitosan(n means degree of polymerization) 1.1.1.2 壳聚糖的物理性质 纯壳聚糖在室温下是一种无定型粉末状固体，呈半透明白色，并且无特殊气味。壳聚糖糖苷键在酸性溶液中会进行水解，也可由于酶、辐射等原因而造成分子链的降解[1]。水解使得壳聚糖可以溶解于某些稀酸溶液，但在水、碱性溶液以及大部分有机溶剂中无法溶解。 壳聚糖是迄今为止在自然界的众多多糖类物质中少数存在的一种带电荷的碱性多糖，。壳聚糖具有许多独特性质，包括良好的透气性、水分吸着性、延展性以及在环境中的生物可降解性等。 壳聚糖已被广泛应用于食品、医药、生物医学、水处理、化妆品等众多不同领域中。不同分子量的壳聚