硫酸镍致大鼠睾丸细胞线粒体及微粒体损伤.docVIP

硫酸镍致大鼠睾丸细胞线粒体及微粒体损伤 目录 TOC \o 1-9 \h \z \u 目录 1 正文 1 文1：硫酸镍致大鼠睾丸细胞线粒体及微粒体损伤 1 1 材料与方法 3 13 方法 3 2 结果 4 3 讨论 5 文2：硫酸镍致大鼠睾丸细胞线粒体及微粒体损伤 5 1; 材料与方法 6 13; 方法 7 2; 结果 8 参考文摘引言： 8 原创性声明（模板） 9 文章致谢（模板） 10 正文 硫酸镍致大鼠睾丸细胞线粒体及微粒体损伤 文1：硫酸镍致大鼠睾丸细胞线粒体及微粒体损伤 Oxidative damage of mitochondria and micriosome in testis cells induced by nickel sulfate in rats Abstract： Objective To explore the mitochondria and microsome of testis cells caused by nickel sulfate in Thirtytwo male Wistar rats with sexual maturation were divided into four groups randomly,and three groups of rats were administrated intraperitoneally with nickel sulfate daily at doses 125,250，500 mg/kg respectively for 30 days,but the control group was administrated with saline in same volume according to the rats mitochondria and microsome of testis cells were prepared by centrifugation technique to detect the levels of lipid peroxidation (LPO),total antioxide capacity (T-AOC),reactive oxygen species (ROS) in The organ coefficient of testis exposed to NiSO4 had no change compared wth the control group(P＜005).The LPO and ROS contents in testis of exposure groups were higher than that of control group(P＞005),but the T-AOC levels of the exposure groups were decreased compared with the control group(P＜001).Conclusion The rats testis damage is related to the enhancement of oxidative stress of mitochondria and microsome caused by nickel sulfate. Key words： nickel sulfate;testis;mitochondria;microcome;oxidative stress 镍是机体生命活动所必需的微量元素，其生物学作用极为广泛，但其化合物又是当前主要的职业危害因素和环境污染物之一。过量镍的摄入可造成机体多系统、多器官损伤〔１〕，同时也是一种比较明确的致癌剂〔2，3〕。动物实验发现，大剂量的镍及其化合物对雄性实验动物生殖功能具有毒性损害作用，镍可透过血-睾屏障，蓄积在睾丸组织中〔4〕，引起睾丸生精代谢障碍，精子生成减少，形态发生改变，畸形率升高〔5〕，并可引起雄性大鼠性激素水平的改变〔6〕。研究发现，镍可致大鼠血清、肌肉、肝脏和肾脏中脂质过氧化物(LPO)含量升高，并引起抗氧化酶活性的改变〔7，8〕。因此，本文从线粒体和微粒体的氧化损伤角度探索镍对睾丸生殖细胞损伤的机制，为镍致雄性生殖毒性的防治提供理论依据。 1 材料与方法 11 实验动物 健康Wistar雄性大鼠，体重180～220 g(兰州大学实验动物中心) 12 试剂和仪器 硫酸镍(NiSO4)，分析纯(西安化学试剂厂)。脂质过氧化物(LPO)、总抗氧化能力(TAOC)、活性氧(ROS)检测试剂盒(南京建成生物工程研究所)。DptimaＴＭ XL100K型高速