As2O3抑制人淋巴瘤Raji细胞增殖及VEGF表达.docVIP

As2O3抑制人淋巴瘤Raji细胞增殖及VEGF表达 目录 TOC \o 1-9 \h \z \u 目录 1 正文 1 文1：As2O3抑制人淋巴瘤Raji细胞增殖及VEGF表达 1 文2：姜黄素对Raji细胞增殖及VEGF表达的影响 7 1 资料与方法 7 2 结果 8 3 讨论 8 参考文摘引言： 9 原创性声明（模板） 10 文章致谢（模板） 10 正文 As2O3抑制人淋巴瘤Raji细胞增殖及VEGF表达 文1：As2O3抑制人淋巴瘤Raji细胞增殖及VEGF表达 Inhibited Proliferation of B-lymphoma Raji Cells and Down-regulated Expression of VEGF by Aenic Trioxide Abstract This study was aimed to investigate the effects of As2O3 on proliferation of B lymphoma Raji cell and to study the expression changes of VEGF mRNA. The modified MTT was adopted to evaluate the effect of As2O3 on proliferation of Raji cells. Semi-quantitative RT-PCR was used to detect the expression of VEGF121 and VEGF165 mRNA in Raji cells exposed to As2O3. The results showed that the As2O3 significantly inhibited the proliferation of Raji cells，the relatiohip between the inhibition rate and the concentratio of As2O3 was dose-and time-dependent. The VEGF121 and VEGF165 mRNA expressio were found in Raji cells，and both were well-matched in expression levels. After treatment of As2O3 at 1 μmol/L for 48 hou，the expression levels of VEGF121 and VEGF165 mRNA were significantly down-regulated and demotrated negative corelation with the time of exposure to As2O3. Otherwise some difference were observed in effects of As2O3 on VEGF121 and VEGF165，the expression of VEGF165 mRNA was down-regulated earlyer and longer than that of VEGF121，while no similar correlation was found in selected concentration groups. It is concluded that As2O3 can significantly inhibit the growth of Raji cells and may exerted its anti-angiogenesis effects by down-regulating the VEGF mRNA expression even in a low concentration for a long term. Key words aenic trioxide; lymphoma; vascular endothelial growth factor (VEGF); angiogenesis 自从As2O3用于急性早幼粒细胞白血病（APL） 治疗 以来，其良好的疗效和特异的作用机制已引起人们关注。多数研究认为诱导凋亡是As2O3的主要作用机制，但也有报道As2O3可下调慢性髓性白血病患者骨髓细胞培养上清液中血管内皮生长因子（VEGF）的表达［1］，因此As2O3的抗肿瘤机制中亦可能包含有抗血管生成效应。本研究在进一步观察As2O3对人淋巴瘤Raji细胞增殖活性影响的基础上，初步探讨其