11种中药对小鼠淋巴细胞活性及腹腔巨噬细胞功能的影响.docVIP

11种中药对小鼠淋巴细胞活性及腹腔巨噬细胞功能的影响 目录 TOC \o 1-9 \h \z \u 目录 1 正文 1 文1：11种中药对小鼠淋巴细胞活性及腹腔巨噬细胞功能的影响 1 1 材料 3 2 方法 4 3 结果 5 4 讨论 6 文2：中药天年饮对衰老大鼠睾丸功能的影响 7 1 材料与方法 9 2 结果 10 3 讨论 11 参考文摘引言： 12 原创性声明（模板） 13 文章致谢（模板） 14 正文 11种中药对小鼠淋巴细胞活性及腹腔巨噬细胞功能的影响 文1：11种中药对小鼠淋巴细胞活性及腹腔巨噬细胞功能的影响 Effect of 11 Traditional Chinese Herbs on Proliferation of Splenic Lymphocyte and Function Phagocytosis of Peritoneal Macrophage Abstract：ObjectiveTo investigate the immune modulatory effect of water extract of 11 traditional Chinese herbs on the proliferation of splenic lymphocyte and function phagocytosis of peritoneal macrophage. Methods The pattern of concanavalin A (ConA) or lipopolysaccharide(LPS) induced proliferative respoe of murine lymphocytes was assessed. Cell proliferation was measured by the XTT (2,3bis［2 4nitro5sulfophenyl］-2H-tetrazolium5carboxanilide assay in the normal cells and the cells treated with ConA or LPS, then measured the proliferation after treated with water extract of 11 traditional Chinese herbs( The final concentration of water extract of 11 traditional Chinese herbs: ，，， μg/ml). Peritoneal macrophage stained red with Neutral Red Dye. The function phagocytosis of peritoneal macrophage after treated with water extract of 11 traditional Chinese herbs for 12 hou was measured by the dose of Neutral Red Dye that they results showed that there was no effect of water extract of 11 traditional Chinese herbs on normal lymphocytes. The water extract of Scutellaria baicaleis had noticeable inhibitory effect on the lymphocytes treated with ConA or LPS. The inhibition of the proliferation of lymphocytes treated with ConA after exposure to Scutellaria baicaleis was dose-dependent, its 50% inhibitory concentration(IC50) was μg/ml. The corrinhibition ratio was 41%, 63%, 70%, 78%. The inhibition of the proliferation of lymphocytes treated with LPS after exposure to Scutellaria baicaleis was dosedependent, too. Its IC50 was μg/ml. The corrinhibiti