中药天年饮对衰老大鼠睾丸生精细胞凋亡及相关因素的影响.docVIP

中药天年饮对衰老大鼠睾丸生精细胞凋亡及相关因素的影响 目录 TOC \o 1-9 \h \z \u 目录 1 正文 1 文1：中药天年饮对衰老大鼠睾丸生精细胞凋亡及相关因素的影响 1 1 材料与方法 3 2 结果 4 3 讨论 5 文2：中药天年饮对衰老大鼠睾丸生精细胞增殖及相关因素的作用 7 1 材料与方法 8 2 结果 9 3 讨论 10 参考文摘引言： 12 原创性声明（模板） 13 文章致谢（模板） 13 正文 中药天年饮对衰老大鼠睾丸生精细胞凋亡及相关因素的影响 文1：中药天年饮对衰老大鼠睾丸生精细胞凋亡及相关因素的影响 Abstract：Objective To observe the effects of traditional Chinese medicine Tiannianyin on apoptosis and related facto of spermatogenic cells in testis of aging rats. Methods40 SD rats were applied in this study and divided into 4 groups at random: normal group, aging model group, Tiannianyin group, negative control group, and each group had 10 rats. The sub-acutely aging model rats were made by injecting D-gal into abdominal cavity continuously. ELISA was used to detect the testosterone content in serum, SP immunohistochemistry to observe the p53 expression and TUNEL to detect apoptosis of spermatogenic cells in testis. ResultsThe testosterone content in serum of aging rats decreased significantly compared with normal rats (P); the p53 positive cells and apoptotic index of aging rats were much higher than those in normal rats (P). Tiannianyin could inhibit the decrease of testosterone content in serum significantly (Tiannianyin group compared with aging model group: P), and decrease the p53 expression and apoptotic index significantly(P, P). ConclusionTraditional Chinese medicine Tiannianyin can improve the spermatogenic function of aging rats, and has fairly anti-aging effects. Key words：Tiannianyin; Aging; Spermatogenic cell; Apoptosis; Testosterone; p53 gene 抗衰老是一个古老的话题，目前国内、外应用的抗衰老方法有很多。中药延缓衰老作用的研究已逐渐深入，在细胞传代、寿命实验、免疫内分泌代谢等领域进行了多方面的研究，揭示了不少药物的抗衰老机理，但各自研究的方面不同。天年饮由刘河间《宣明论方》何首乌丸加味而成，由何首乌、肉苁蓉、怀牛膝等组成，具有补肝肾、益精血、延年益寿之功效。本研究采用D-半乳糖致亚急性衰老的大鼠模型，观察天年饮对睾丸生精细胞凋亡及相关因素的影响，探讨天年饮延缓衰老的作用机制。 1 材料与方法 动物的选择及分组 成年雄性SD大鼠40只，体重180~250 g（由河北医科大学实验动物中心提供，合格证书：医动字第04057号）。将大鼠随机分为4组，正常组、衰老模型组、天年饮用药组和阴性对照组，每组10只。其中模型组、用药组、阴性对照组均采用D-半乳糖连续腹腔注射（按200 mg#12539;kg1-#12539;d-1给药，1次/d，连续40 d）制作亚急性衰