色谱指纹图谱分析技术用于鉴别中药玄参产地的研究.docVIP

色谱指纹图谱分析技术用于鉴别中药玄参产地的研究 目录 TOC \o 1-9 \h \z \u 目录 1 正文 1 文1：色谱指纹图谱分析技术用于鉴别中药玄参产地的研究 1 1 器材与方法 3 2 结果 5 3 讨论 7 文2：延胡索产地醋煮工艺的研究 10 1 仪器与试药 11 2 方法与结果 12 2. 1 含量测定方法的建立［3］ 12 3 讨论 16 参考文摘引言： 17 原创性声明（模板） 18 文章致谢（模板） 18 正文 色谱指纹图谱分析技术用于鉴别中药玄参产地的研究 文1：色谱指纹图谱分析技术用于鉴别中药玄参产地的研究 Abstract：ObjectiveTo identify the Habital of Radix Scrophulariae with Chromatographic cotituents were extracted from ten batches of sample of Radix Scrophulariae (Scrophularia ningpoeis Hemsl.), which collected from Chongqing Wulong county, and evaluated by HPLC. Chromatographic conditio was Kromasil TM C18 column with methanol-1% glacial acetic acid as mobile phase, the detection wavelength was 290 nm, the flow rate was ml·min-1, and column temperature was 30℃.ResultsMany active cotituents that extracted from ten batches of sample of Radix Scrophulariae (Scrophularia ningpoeis Hemsl.), which collected from Chongqing Wulong county, were evaluated by HPLC. The models of standard HPLC Fingerprint of Radix Scrophulariae were established; Taking the cinnamic acid as the reference peak, the relative time and the relative peak area ration of various characteristics peak were calculated, and the sample from Zhejiang province could be distinguished by this model and the relative peak area ratio of the characteristic study is good for the standard planting and quality control of Radix scrophulariae. Key words：Radix Scrophulariae（Scrophularia ningpoeis Hemsl.）; Cinnamic acid； HPLC； Fingerprint； Quality control 玄参科植物玄参Scrophularia ningpoeis Hemsl. 的干燥根具有凉血滋阴、泻火解毒的功效，临床上用于 治疗 热病伤阴，舌绛烦渴，温毒发斑，津伤便秘，骨蒸劳嗽，目赤，咽痛，瘰疬，白喉，痈肿疮毒［1］。玄参含哈巴苷、哈巴苷元、桃叶珊瑚苷、6-对甲基梓醇、浙玄参苷甲、乙等环烯醚萜类化合物及生物碱、植物甾醇、油酸、硬脂酸、葡萄糖、天冬酰胺、微量挥发油等［2］。《 中国 药典》2005年版Ⅰ部对玄参的质量控制采用HPLC法测定其中哈巴俄苷的含量［1］。众所周知，单一指标性成分的含量测定往往难以反映中药整体质量，而指纹图谱具有系统性、特征性和重现性特点，能较全面反映中药品质，现已成为中药质量评价和控制研究的热点之一［3～5］。本文以具有轻泻、抗细菌及真菌等作用的肉桂酸 ［6］作为指标成分，采用HPLC法构建不同产地玄参药材的指纹特征谱，为追踪不同产地的生药来源、保证中成药的质量可控、建立可行的质量控制方法奠定了基础。 1 器材与方法 仪器Wate 2695高效液相色谱仪，Wate 2996 DAD二极管阵列检