前列腺组织二氢睾酮结合蛋白与性激素作用的研究.docVIP

前列腺组织二氢睾酮结合蛋白与性激素作用的研究 目录 TOC \o 1-9 \h \z \u 目录 1 正文 1 文1：前列腺组织二氢睾酮结合蛋白与性激素作用的研究 1 1 材料和方法 2 2 结果 4 3 讨论 5 文2：TUVRP与TURP术中前列腺组织耗损量的比较 6 1 资料与方法 6 2 结果 8 3 讨论 8 参考文摘引言： 9 原创性声明（模板） 11 文章致谢（模板） 11 正文 前列腺组织二氢睾酮结合蛋白与性激素作用的研究 文1：前列腺组织二氢睾酮结合蛋白与性激素作用的研究 【Abstract】 Objectives This experiment is to quantify the total DHT (Dihydrotestosterone)-binding ability of DHT-binding protei in prostate by assaying the DHT-binding capacity, and to study the change of DHT-binding capacity causing by the combination of T（Testosterone）, E2(Estrodiol), Po(Progestosterone) and DHT-binding protein, and to analyze preliminarily the effects of sex hormone on DHT-binding protein of To obtain cytosolic fracio from 20 normal tissues taken from accident-death corpses without serious diseases, and then to remove all the endogenous hormone from the cytosolic fracio by ether assay the content of the bound 3H –DHT. To add the same dose of T、E2、Po to compete agait 3H –DHT for DHT protein, and then to add 10 times dose of T、E2、Po to compete with 3H –DHT for DHT protein. Results The alone DHT-binding capacity of the cytosolic fractio in prostate is (±)nmol/g wet tissue. T and E2 have more obvious effect on reducing the DHT-binding capacity of the cytosolic fraction while Po has less effect on reducing the DHT-binding capacity. Conclusio There are many kinds of DHT-binding protei in the cytosolic fraction in prostate tissues and their mechanisms are different. The protei have strong DHT-binding capacity, and they interact with T、E2、Po to affect the DHT state in the prostate tissues. 【Key words】 Prostate Androgen Estrogen Progestosterone Androgen-binding protein 前列腺组织中存在多种二氢睾酮（Dihydrotestosterone，DHT）结合蛋白［１］，这些蛋白的性质和生理作用尚不十分明确。作者自2001年2月至2007年5月实验设计测定DHT结合容量来描述这些结合蛋白总的DHT结合能力，并以睾酮(CT)、雌二醇(E2)、孕酮(PO)与DHT竞争结合蛋白的作用研究正常前列腺DHT结合容量的变化，并初步分析DHT结合蛋白与性激素作用的性质。 1 材料和方法 前列腺组织标本 20例正常前列腺组织取自无严重疾患的健康男性尸体，年龄22～44岁，平均岁。组织块获取距临床诊断死亡时间＜15min。所有组织均经病理证实。组织获取后以冰生理盐水洗净血迹，保留腺体实质部分，滤纸吸干，置