贫铀长期植入对大鼠肾脏损伤效应的研究.docVIP

贫铀长期植入对大鼠肾脏损伤效应的研究 目录 TOC \o 1-9 \h \z \u 目录 1 正文 1 文1：贫铀长期植入对大鼠肾脏损伤效应的研究 1 1 材料与方法 3 2 结果 4 3 讨论 6 文2：黄芪皂苷和黄芪多糖对大鼠肾脏系膜细胞增殖的影响 8 1 器材 8 2 方法与结果 10 3 讨论 11 参考文摘引言： 12 原创性声明（模板） 13 文章致谢（模板） 13 正文 贫铀长期植入对大鼠肾脏损伤效应的研究 文1：贫铀长期植入对大鼠肾脏损伤效应的研究 Abstract：Objective To study the toxic effects on rat kidney after long term depleted uranium(DU) Twenty adult male rats were implanted DU plates as implanted morphology changes of kidneys were observed by electronmicroscopy and microscopy after 3,6,12,18 months cytokines such as TGF-β1 and Smad were analyzed by immunofluorescence and Western function and collagen staining were also At different time-point,the morphological structure observation showed that DU exposure can cause the kidney fibrosis among which glomerulus pyknosis,tubule atrophy and regional renal intetitial fibrosis became aggravated with longer analysis and collagen staining showed that these cytokines and collagen distribution primarily located on periphery of nephric tubule,and seldom on glomerulus if β1 and Smad are important facto for tissue fibrosis. Western Blot analysis showed that Traforming Growth Factor beta 1(TGF-β1) and Smad in kidney all increased after DU After long term DU exposure,the rats kidneys showed morphology and ultrastructure changes at different β1 and Smad both took part in the process of fibrosis. Key words:depleted uranium；kidney；fibrosis；toxicity 贫铀(Depleted Uranium，DU)是铀原料经浓缩提取235U以后所产生的副产品，因其235U含量低于天然铀故而得名。目前DU合金被广泛应用于各类民用和军事项目。贫铀既具有放射毒性，又具有化学毒性，由于其高传能线密度，特定组织器官蓄积以及半衰期长的特点，人员因嵌入、吸入或食入贫铀后，会对人体产生严重损害作用。损害作用与接触的剂量、时间以及接触者的年龄、性别、体质、饮食等有关［１］。肾脏是铀的主要蓄积器官和排泄器官，进入机体的铀绝大部分进入肾脏并通过尿液排出体外，因此，肾脏在铀对机体的毒性危害中占重要地位。铀染毒后可引起典型的急性肾功能衰竭。肾脏纤维化与慢性肾功能衰竭的进展关系十分密切，几乎所有的慢性进行性肾脏疾病都是肾纤维化破坏过程的结果。因此，本研究拟通过大鼠长期低剂量接触贫铀，检测肾脏功能，肾脏的超微结构和组织病理形态学观察，胶原染色，Western Blot分析以及免疫组化检测。研究贫铀对大鼠肾脏可能存在的损伤效应，以TGF-β 1为代表的组织纤维化相关细胞因子在肾纤维化进程中所起的作用，对贫铀可能引起的肾脏损伤及其机理进行初步研究。 1 材料与方法 DU植入模型的建立 采用初成年雄性Wistar大鼠，体质量为150～200 g，20只，右后肢贫铀片植入，DU片组成、大小及植入方法见 文