慢性胃炎异型增生和胃癌组织中baxiNOS表达与幽门螺杆菌感染的关系临床医学.docVIP

慢性胃炎异型增生和胃癌组织中baxiNOS表达与幽门螺杆菌感染的关系临床医学 目录 TOC \o 1-9 \h \z \u 目录 1 正文 1 文1：慢性胃炎异型增生和胃癌组织中baxiNOS表达与幽门螺杆菌感染的关系临床医学 1 1 资料与方法 3 2 结果 4 3 讨论 4 文2：慢性胃炎异型增生胃癌幽门螺杆菌感染与iNOS表达关系的研究临床医学 5 1 资料与方法 6 2 结果 7 3 讨论 7 参考文摘引言： 8 原创性声明（模板） 9 文章致谢（模板） 9 正文 慢性胃炎异型增生和胃癌组织中baxiNOS表达与幽门螺杆菌感染的关系临床医学 文1：慢性胃炎异型增生和胃癌组织中baxiNOS表达与幽门螺杆菌感染的关系临床医学 【ABSTRACT】 Objective: To investigate the expression of bax,iNos in different gastric mucosa lesion and their relatiohip with the infection of HP. Methods: The total gastric tissues and the corresponding blood serum had been collected from 102 cases, including 15 cases with inflammatory gastric mucosa,20 cases with gastric epithelial dysplasia and 65 cases with gastric carcinoma. In all cases,immunohistochemical streptavidiperoxidase(SP)method was used to assess the expression of bax and iNos; HP was detected by Gram staining and ELISA in tissues and blood serum respectively. Results: The positive rate of HP was 7/15(%)in inflammatory gastric mucosa, 15/22(%)in gastric epithelial dysplasis and 34/65(%)in gastric of both bax and iNos had a positive relatiohip with HP infection; In HP positive group the expression of bax and iNos were higher than that in HP negative group. Conclusion: Expression of bax and iNos showed significance relatiohip with the infection of HP,it might induce proliferation and apoptosis of gastric epithelial cells,this might play an important role in the carcinogenesis of gastric carcinoma. 【KEY WORDS】 Inflammatory gastric mucosa; Gastric epithelial dysplasia; Gastric cancer; HP; bax; iNOS 胃癌的发生大部分由正常粘膜逐渐演变为癌前病变，并发展成癌[1] ,粘膜上皮异型增生已被公认为癌前病变。HP感染被证实是胃粘膜病变最重要的启动因素[2]， 一氧化氮（NO）作为一种自由基能直接损伤细胞DNA，诱导细胞凋亡[3]，bax 基因蛋白是bcl 2基因家族中促进细胞凋亡的基因，三者之间的关系尚无 文献 报导。为探讨HP感染与iNOS及bax基因蛋白在胃癌发生过程中的表达情况，我们采用SP免疫组化法及酶联免疫法检测102例胃组织标本，旨在探讨胃癌的发生、发展过程中的一些外界和内在因素作用的机制。 1 资料与方法 一般资料 我院2002～2004年102例胃组织标本，经内镜及组织病理诊断65例胃癌、22例异型增生和15例慢性胃炎；男性66例，女性36例；年龄28～72岁，中位年龄岁。标本取材后经4%甲醛固定，常规石蜡包埋，厚5μm连续切片。普通光镜下进行确诊，同时采集102例标本血清，应用酶联免疫法进行血清