动脉粥样硬化形成过程中内源性一氧化碳和一氧化氮的变化及其相互关系临床医学.docVIP

动脉粥样硬化形成过程中内源性一氧化碳和一氧化氮的变化及其相互关系临床医学 目录 TOC \o 1-9 \h \z \u 目录 1 正文 1 文1：动脉粥样硬化形成过程中内源性一氧化碳和一氧化氮的变化及其相互关系临床医学 1 1 材料与方法 3 2 结 果 4 3 讨 论 5 文2：抵当芪桂汤对链脲佐菌素诱导糖尿病大鼠血清内皮素1和一氧化氮的 7 1 材料与仪器 9 2 方法 9 3 结果 10 参考文摘引言： 10 原创性声明（模板） 12 文章致谢（模板） 13 正文 动脉粥样硬化形成过程中内源性一氧化碳和一氧化氮的变化及其相互关系临床医学 文1：动脉粥样硬化形成过程中内源性一氧化碳和一氧化氮的变化及其相互关系临床医学 【Abstract】 Objective To study the change of endogenous carbon monoxide (CO) and nitric oxide (NO) in rabbit aorta with atherosclerosis(AS) induced by high cholesterol diet and the inffluence of CO and NO on AS process. Methods The rabbits were given % cholesterol diet (Ch group) or % cholesterol diet plus hemin (15 mg·kg-1·ｄ-1, ip., Hm group) or zincprotoporphyrin IX (ZnppIX, 45 μmol·kg-1·ｄ-1, ip., Zn group) or Larginine(% in drinking water, Arg group) or Lnitroarginine methylester(% in drinking water, LNAME group), each group had 8 rabbits. After 12 w, the levels of serum lipids(TC, TG, LDLC, HDLC, oxLDL) and the contents of aortic CO and NO,NO synthases(cNOS) and heme oxygenase(HO) activity were assessed, the aortic AS was measured by image analyse system. Results High cholesterol diet markedly enhanced the levels of serum TC,TG, LDLC and oxLDL(all P＜).Compared with those of control group, the contents of aortic NO and cNOS were decreased markedly, the content of CO and the activity of HO were increased markedly(all P＜) in Ch group, and the aortic plaques area was (±)%. Hemin injection increased markedly the activities of aortic HO and CO, the aortic plaques area was (±)%. ZnppIX injection made the opposite results, the aortic plaques area was (±)%. Larginine increased markedly the activities of aortic cNOS and CO, the aortic plaques area was (±)%, LNAME made the opposite results, the aortic plaques area was (±)%. Conclusio Aortic NOS/NO system are impaired significantly in AS reciprocal correlation of HO/CO and NOS/NO systems in AS could play the protective role agait AS lesion. 【Key words】 Atherosclero