抑郁症模型大鼠睾酮水平的研究.docVIP

抑郁症模型大鼠睾酮水平的研究 目录 TOC \o 1-9 \h \z \u 目录 1 正文 1 文1：抑郁症模型大鼠睾酮水平的研究 1 1 材料与方法 2 2 结 果 4 3 讨 论 5 文2：针刺对抑郁症模型大鼠海马区单胺类神经递质的影响 6 1 材料与方法 7 2 结果与分析 10 3 讨论 11 参考文摘引言： 13 原创性声明（模板） 14 文章致谢（模板） 15 正文 抑郁症模型大鼠睾酮水平的研究 文1：抑郁症模型大鼠睾酮水平的研究 【Abstract】 Objective To investigate the changes of the level of testosterone in two types of depressed rats, and study the relatiohip between testosterone and depressive disorder. Methods The rats was exposed to chronic mild stress (CMS) such as food deprivation, water deprivation, continuous lighting etc for 21 days. The male rats which born for 8 days were administered chlomipramine (CLI) by intraperitoneal injection till 21 days, then two months later behavioral test and other experimental process were performed. The level of testosterone was detected by radioimmunity. Results The immobility time of two types of the depressed rats were longer than those of control rats (P) and rearing time were shorter than those of control rats (P) in forcedswimming test. The crossing times and rearing times of CMS rats were decreased after stress in openfield test (P). The crossing times of CLI rats were increased (P) and the rearing times of CLI rats were decreaed (P) in openfield test. The levels of testosterone of testicle and serum in CLI rats were both increased (P). But the levels of testosterone of testicle and serum in CMS rats were both decreased (P). Conclusio There are sexual hormone parasecretion in depressed rats. The changes of testosterone is related with the type of depressive disorder. 【Key words】 Depressive disorder; Testosterone; Testicle; Rats 抑郁症的发生与性激素分泌水平是否具有相关性报道不一〔1〕，本实验主要观察外源性抑郁症大鼠模型和内源性抑郁症大鼠模型睾丸组织及血清内睾酮水平的变化，为探讨抑郁症对机体性激素水平及性功能的影响奠定基础。 1 材料与方法 实验动物分组及模型制备 动物 均采用健康雄性清洁级Wistar大鼠，体重180～210 g及出生第8天的雄性幼鼠。由吉林大学白求恩医学院动物实验中心提供。 外源性抑郁症大鼠模型 造模根据 文献 〔2〕的方法稍加改进。成年雄性Wistar大鼠，首先用强迫游泳实验进行行为评分，选择得分相近的动物20只，随机分为正常组10只;其余10只。每天随机实施禁食、禁水、隔离、光照等不同的慢性刺激，持续21 d，为外源性抑郁症大鼠模型(CMS组) 内源性抑郁症大鼠模型 给予新生第8天的雄性幼鼠腹腔注射新鲜配制氯咪帕明