哮喘儿童外周血T淋巴细胞FasFasL的表达及其意义药学.docVIP

哮喘儿童外周血T淋巴细胞FasFasL的表达及其意义药学 目录 TOC \o 1-9 \h \z \u 目录 1 正文 1 文1：哮喘儿童外周血T淋巴细胞FasFasL的表达及其意义药学 1 1 对象与方法 2 2 结果 3 3 讨论 4 文2：慢性乙型病毒性肝炎患者外周血T淋巴细胞亚群CD27和CD28的表达 5 1 材料与方法 7 2 结果 8 3 讨论 9 参考文摘引言： 11 原创性声明（模板） 12 文章致谢（模板） 13 正文 哮喘儿童外周血T淋巴细胞FasFasL的表达及其意义药学 文1：哮喘儿童外周血T淋巴细胞FasFasL的表达及其意义药学 Abstract：ObjectiveTo investigated the expression of Fas/FasL on peripheral blood T cells in asthmatic children and to explore the relatiohip between its clinical significance. MethodsLymphocytes were isolated with ficoll-hypaque gradient method and T lymphocytes were further purified with nylon pili. Expression of Fas/FasL was detected by immunofluoresence of Fas on T cells in 21 cases of the stage of attack (±) and in 15 cases of remission stage (±) were remarkably lower than those in 25 healthy children (±) (P). Expression of FasL on T cells in the stage of attack (±) was significantly increased compared with normal control group (±) (P) but those in remission patients (±) was not significantly changed. There was a remarkable negative relatiohip between expression of Fas and percentages of peripheral blood lymphocyte (r=-, P).ConclusionThe apoptosis of peripheral blood T lymphocytes from the children with asthma both in stage of attack and remission are decreased. The inhibited apoptosis might be one of reaso for the chronic peistent inflammation in the airways in asthmatic children and suggests that it is necessary to continue therapy in remission stage. Key words： Asthma； Child； Lymphocyte ； Fas； FasL 支气管哮喘以可逆性气道阻塞、气道高反应性、气道炎症为其三大主要特征，其中以气道炎症为最主要的病理变化，并决定气道阻塞和气道高反应性的程度［1］。这种气道慢性变态反应性炎症是以嗜酸细胞、淋巴细胞及肥大细胞浸润为主的。最近研究表明，淋巴细胞凋亡与激活的调节失衡可能起到重要作用［2］。本课题组近期对哮喘儿童外周血T淋巴细胞Fas/FasL表达的变化进行了研究，以探讨其在哮喘中的作用。 1 对象与方法 对象哮喘发作期21例，男14例，女7例，年龄3~12岁；缓解期15例，男9例，女6例，年龄3~12岁；所有患儿均符合全国哮喘诊断标准［3］，均于采血前1周内未使用糖皮质激素。同期体检健康儿童25例作为对照，男14例，女11例，年龄3~12岁。 方法 外周血淋巴细胞分离：无菌取静脉血肝素抗凝，加等量Hanks液稀释，用Ficoll淋巴细胞分离液分离，用含10%胎牛血清（FBS, Sigma产品）的RpMI 1640培养液（GIBCO产品）配成细胞悬液，细胞浓度为5×109/L，于培养瓶中37 ℃贴壁培养2