补肾活血方对血管性痴呆小鼠行为学及脑海马自由基代谢的影响临床医学.docVIP

补肾活血方对血管性痴呆小鼠行为学及脑海马自由基代谢的影响临床医学 目录 TOC \o 1-9 \h \z \u 目录 1 正文 1 文1：补肾活血方对血管性痴呆小鼠行为学及脑海马自由基代谢的影响临床医学 1 1 材料与方法 3 2 结 果 4 3 讨论 5 文2：补肾活血方对小鼠自身免疫性卵巢功能衰退的防治作用 6 1 材料与方法 8 2 结果 10 3 讨论 11 参考文摘引言： 12 原创性声明（模板） 13 文章致谢（模板） 14 正文 补肾活血方对血管性痴呆小鼠行为学及脑海马自由基代谢的影响临床医学 文1：补肾活血方对血管性痴呆小鼠行为学及脑海马自由基代谢的影响临床医学 【Abstract】Objective To probe the effects of bushenfang, huoxuefang, and bushenhuoxuefang on the abilities of learning and memory and free radical metabolism of vascular dementia (VD) mice. Methods The models of VD mice were established by cerebral ischemia reperfusion, the praxiological changes were observed by water maze and the activity of superoxide dismutase (SOD) and the content of malondialdehyde (MDA) in hippocampi were measured. Results Compared with those in normal group, the abilities of learning and memory in VD model mice were reduced, which showed extending the time for swimming the whole process, and increasing the time of mistakes(P＜), and the activity of SOD in hippocampi was decreased and the content of MDA was increased(P＜). Compared with those in model group, the learning and memory performance records were improved (P＜), and the activity of SOD was increased and the content of MDA was decreased (P＜ or P＜) in hippocampi in each treatment group. The efficacy of bushenhuoxue group was more excellent than that of other treatment groups(P＜ or P＜). Conclusio The bushen, huoxue, and bushenhuoxue traditional Chinese medicine could improve free radical metabolism and the learning and memory capabilities, and the two kinds of traditional Chinese medicine have synergistic actio. 【Key words】Bushenhuoxue；Vascular dementia; Praxiology; Free radical metabolism 血管性痴呆(VD)是因脑血管病所致的智能及认知障碍，为老年人常见病、多发病。补肾中药和活血中药为临床治疗VD最常用药物，具有公认的疗效〔1〕，但临床所用复方多为多种治法综合而成，补肾法和活血法是否有协同作用，目前尚未见报道。为了探讨不同治法对于VD的作用效果及其协同关系，本研究采用反复脑缺血再灌注的方法复制VD小鼠模型，观察补肾活血类中药对模型小鼠行为学及自由基代谢的影响，现报道如下。 1 材料与方法 实验动物 清洁级昆明小鼠80只，雌雄各半，体重25～30 g，由河南省中兴实验动物养殖中心提供。 实验用药 补肾方：由何首乌、益智仁、胡桃肉、人参组成。活血