葶苈生脉饮对压力负荷大鼠心肌组织MMP9及TIMP1表达的影响临床医学.docVIP

葶苈生脉饮对压力负荷大鼠心肌组织MMP9及TIMP1表达的影响临床医学 目录 TOC \o 1-9 \h \z \u 目录 1 正文 1 文1：葶苈生脉饮对压力负荷大鼠心肌组织MMP9及TIMP1表达的影响临床医学 1 1 材料与方法 3 2 结 果 5 3 讨 论 5 文2：低分子肝素对COPD模型大鼠MMP9及TIMP1影响医学 7 1 材料与方法 8 2 结 果 9 3 讨 论 11 参考文摘引言： 12 原创性声明（模板） 13 文章致谢（模板） 14 正文 葶苈生脉饮对压力负荷大鼠心肌组织MMP9及TIMP1表达的影响临床医学 文1：葶苈生脉饮对压力负荷大鼠心肌组织MMP9及TIMP1表达的影响临床医学 【Abstract】 Objective To observe the effects of Tinglishengmaiyin on the expressio of matrix metalloproteinases9 (MMP9) and tissue inhibitor of metalloproteinases (TIMP1) in the myocardiac tissue of rats with congestive heart failure (CHF) induced by over loaded pressure. Methods The model rats with CHF were established by suprarenal aortic cotriction operation and divided into 5 groups randomly: sham operated, model control, Tinglishengmaiyin high, low dose groups and Xinbaowan group. The MS4000U biological signal collecting system was used to record and analyze the changes of LVSP, LVEDP and±dp/dtmax. Immunohistochemical method was used to observe the expressio of MMP9 and TIMP1. Results In model control group, the LVEDP, dp/dtmax, the expression of MMP9 increased (P or P) and the LVSP, +dp/dtmax, the expression of TIMP1 decreased (P or P). Compared with model control group， LVEDP, dp/dtmax, the expression of MMP9 decreased (P or P), LVSP,+dp/dtmax, the expression of TIMP1 increased (P or P) in the treatment groups. High dose group was significantly superior to low dose group and Xinbaowan group (P). Conclusio Tinglishengmaiyin can adjust the expressio of MMP9 and TIMP1, which may be one of the mechanisms of reveing myocardial model and improving heart function to treat CHF. 【Key words】 Tinglishengmaiyin; Heart failure; MMP9; TIMP1 心室重塑是充血性心力衰竭发生 发展 的重要病理生理过程，是由于一系列复杂的分子和细胞机制导致的心肌结构、功能变化。近年研究发现，基质金属蛋白酶(MMPs)及内源性基质金属蛋白酶组织抑制因子(TIMPs)的表达变化在心室重塑及心力衰竭的发展过程中具有显著作用〔1〕 。但有关中药对CHF时MMPs及TIMPs表达变化影响的报道较少。葶苈生脉饮是根据中医理论及多年临床实践研制出的治疗CHF的效方。为了进一步阐明其作用机制，本文观察了其对压力超负荷致充血性心力衰竭大鼠模型心肌组织MMP9及TIMP1表达的影响。 1 材料与方法 药物 葶苈生