广西长寿地区人群白细胞DNA总体甲基化水平研究.docVIP

广西长寿地区人群白细胞DNA总体甲基化水平研究 目录 TOC \o 1-9 \h \z \u 目录 1 正文 1 文1：广西长寿地区人群白细胞DNA总体甲基化水平研究 1 1 材料与方法 3 2 结果 5 3 讨 论 6 文2：DNA低氧与DNA甲基化预适应 8 1 DNA 9 2 DNA 甲基化 9 3 DNA 低氧预适应 11 4 DNA 甲基化与低氧预适应的关系 12 参考文摘引言： 14 原创性声明（模板） 15 文章致谢（模板） 15 正文 广西长寿地区人群白细胞DNA总体甲基化水平研究 文1：广西长寿地区人群白细胞DNA总体甲基化水平研究 【Abstract】Objective To compare the total methylation level of the leukocytes from peo living in the longevity district Bama county of Guangxi province with from general district, and study possible relatiohip between the longevity and DNA methylation. Methods 199 healthy individuals in the longevity villages of Bama were selected as case group and another 163 healthy individuals as control group living in general district. 2 ml vein blood was obtained from the subjects to extract DNA and then detect the concentratio of the dC and 5dmC with the HPLC and calculate the value of 5dmC/(dC+5dmC) which represents the subjects total methylation level. Results The total methylation level declined with aging in control and case groups by - 3 and - 7 respectively. Age was negatively correlated to 5dmC/(dC+5dmC) value (r=- 2 and - 6 respectively). The total methylation level of the case group was higher than that of control group(P＜). Analyzing in details, in the year of 10～, 20～, 40～, 50～ and 60～，5dmC/(dC+5dmC) value in case group was higher than that of control group. Conclusio There is relatiohip between individuals longevity in Bama county and their higher total DNA methylation level. 【Key words】Bama County; Longevity; DNA total methylation level 衰老是由一系列基因激活和阻抑及其通过各自表达产物相互作用的结果。DNA甲基化是有核细胞特有的复制后修饰，与肿瘤、遗传病、自身免疫性疾病、衰老的发生密切相关〔1〕。研究表明多数动物的组织或器官基因组中5甲基胞嘧啶(5dmC) 含量随年龄增加而下降〔2〕，Wilson等〔3〕发现培养的人二倍体成纤维细胞中5dmC含量随培养代数增加而降低，说明DNA甲基化程度与细胞寿命有关。Golbus等〔4〕发现36岁以上组人群T细胞基因组中5dmC含量较35岁以下组低约11%，且下降速度存在个体间的差异 〔5〕。以上研究说明基因组DNA甲基化水平降低的趋势在衰老中普遍存在的。 广西巴马县是世界知名的长寿之乡，人群长寿现象自古有之，据全国第五次人口普查，全县共有百岁老人74名，占总人口的31/105，90岁～99岁老人173人，占总人口的75/105，本研究旨在探讨巴马县人群DNA总体甲基化水平，以揭示DNA甲基化与人类长寿之间