米诺环素对衰老痴呆大鼠脑组织eNOSiNOS表达的影响临床医学.docVIP

米诺环素对衰老痴呆大鼠脑组织eNOSiNOS表达的影响临床医学 目录 TOC \o 1-9 \h \z \u 目录 1 正文 1 文1：米诺环素对衰老痴呆大鼠脑组织eNOSiNOS表达的影响临床医学 1 1 材料与方法 3 2 结果 6 3 讨论 7 文2：淀粉样前体蛋白酶与β淀粉样蛋白在衰老痴呆大鼠脑组织中的表达临床医学 8 1 材料与方法 9 2 结 果 10 3 讨 论 11 参考文摘引言： 12 原创性声明（模板） 13 文章致谢（模板） 14 正文 米诺环素对衰老痴呆大鼠脑组织eNOSiNOS表达的影响临床医学 文1：米诺环素对衰老痴呆大鼠脑组织eNOSiNOS表达的影响临床医学 【Abstract】Objective To investigate the effect of minocycline on the expressio of eNOS and iNOS in brain of aged dementia rat，and study its neuroprotection mechanism for aged dementia. Methods Wistar rats were randomly divided into sham, aged dementia model and minocycline treatment groups. eNOS and iNOS were measured by immunohistochemistry and ELISA. Learning memory was measured by praxiology. Results Shuttle box and morris water maze tests in minocycline treatment and aged model groups were more significantly different than those of sham group (P＜). Shuttle box and morris water maze tests in minocycline treatment group were more significantly different than those of sham groups (P＜). The expression of iNOS in minocycline treatment group was lower and the expression of eNOS was higher than that of model group (P＜). The expressio of eNOS, iNOS in minocycline treatment and model groups were higher than those of sham group (all P＜). Conclusio Minocycline can decrease the expression of iNOS and increase the expression of eNOS in aged dementia. Minocycline can play such a neuroprotection role in aged dementia rats by restraining oxidative stress action. 【Key words】Dementia; Minocycline; Nitricoxide synthas 一氧化氮合酶(NOS)是催化一氧化氮(NO)生物合成的酶，广泛存在于各种类型的细胞中。内皮源性一氧化氮合酶(eNOS) 可以激活血管平滑肌细胞中的可溶性鸟苷酸环化酶，使胞内cAMP浓度升高，从而起到扩张血管、抑制血小板和白细胞黏附、聚集的作用，发挥神经保护功能〔1〕。诱导性一氧化氮合酶(iNOS) 主要分布于巨噬细胞、炎性中性粒细胞、血管平滑肌细胞、内皮细胞、小胶质细胞和星型细胞等，一旦合成就不断地产生NO，直至底物耗尽，过量产生的NO可通过活性氮、氧化应激效应对脑组织造成损害〔2〕。米诺环素作为四环素类药物中一种有效的神经细胞保护剂，在抑制神经炎症〔3〕、抑制小胶质细胞激活〔4〕、抑制基质金属蛋白酶表达〔5，6〕、抑制活性氧产物和氧化应激的发生〔7，8〕、抑制细胞调亡〔9〕过程等发挥脑保护作用。本研究通过观察衰老痴呆大鼠动物模型脑组织eNOS和iNOS的表达，探索NOS在衰老痴呆中的发病机制，并采用米诺环素对衰老痴呆大鼠进行干预，探讨米诺环素对衰老痴呆脑保护作用的机